MiniCORVET is a Vps8-containing early endosomal tether in drosophila

Péter Lőrincz, Zsolt Lakatos, Ágnes Varga, Tamás Maruzs, Zsófia Simon-Vecsei, Z. Darula, Péter Benkő, Gábor Csordás, Mónika Lippai, I. Andó, Krisztina Hegedűs, Katalin F. Medzihradszky, Szabolcs Takáts, G. Juhász

Research output: Contribution to journalArticle

23 Citations (Scopus)


Yeast studies identified two heterohexameric tethering complexes, which consist of 4 shared (Vps11, Vps16, Vps18 and Vps33) and 2 specific subunits: Vps3 and Vps8 (CORVET) versus Vps39 and Vps41 (HOPS). CORVET is an early and HOPS is a late endosomal tether. The function of HOPS is well known in animal cells, while CORVET is poorly characterized. Here we show that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog. We find that Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes. Surprisingly, Vps11 deletion causes enlargement of endosomes, similar to loss of the HOPS-specific subunits Vps39 and Lt/Vps41. We thus identify a 4 subunitcontaining miniCORVET complex as an unconventional early endosomal tether in Drosophila.

Original languageEnglish
Article numbere14226
Issue numberJUNE2016
Publication statusPublished - Jun 2 2016


ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Lőrincz, P., Lakatos, Z., Varga, Á., Maruzs, T., Simon-Vecsei, Z., Darula, Z., Benkő, P., Csordás, G., Lippai, M., Andó, I., Hegedűs, K., Medzihradszky, K. F., Takáts, S., & Juhász, G. (2016). MiniCORVET is a Vps8-containing early endosomal tether in drosophila. eLife, 5(JUNE2016), [e14226].