Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro

O. Toldi, Gábor Gyulai, Éva Preininger, E. Várallyay, Miklós Fári, E. Balázs

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An in vitro method for the initiation of minibeet development from derooted sugarbeet seedlings was developed. The surface sterilized seeds were germinated for 25 days on sugar-free Murashige and Skoog medium containing half-strength nutritive elements, 10 mg l-1 thiamine, 0.1 mg l-1 gibberellic acid and 7.0 g l-1 agar. After germination, seedlings of six different genotypes were derooted at the root neck and incubated for 90-120 days on minibeet initiation medium containing double-strength Murashige and Skoog elements, B5 vitamins, 100 mg l-1 activated charcoal, 30 g l-1 d-glucose, 6-benzylaminopurine (0.2 mg l-1) and abscisic acid (0.2 mg l-1). Without subculturing, minibeets developed on 20-40% of five different genotypes of seedlings. The developed minibeets were characterized structurally and functionally by means of a histological study and relative sucrose accumulation. Minibeets were compared to tuber-like formations which showed neither polycambial structure nor relative sucrose accumulation. Minibeets gave rise to plants after potting in the greenhouse.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalPlant Science
Volume97
Issue number2
DOIs
Publication statusPublished - 1994

Fingerprint

N-benzyladenine
Beta vulgaris
Seedlings
sugar beet
Sucrose
Pantothenic Acid
Abscisic Acid
seedlings
Charcoal
Thiamine
Greenhouses
Genotype
Sugars
sucrose
Agar
Seed
pantothenic acid
root crown
genotype
activated carbon

Keywords

  • Abscisic acid
  • Beta vulgaris L
  • Minibeet
  • Polycambial structure
  • Storage organ
  • Sucrose accumulation

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro. / Toldi, O.; Gyulai, Gábor; Preininger, Éva; Várallyay, E.; Fári, Miklós; Balázs, E.

In: Plant Science, Vol. 97, No. 2, 1994, p. 217-224.

Research output: Contribution to journalArticle

Toldi, O. ; Gyulai, Gábor ; Preininger, Éva ; Várallyay, E. ; Fári, Miklós ; Balázs, E. / Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro. In: Plant Science. 1994 ; Vol. 97, No. 2. pp. 217-224.
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