mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila

C. Fiona Cullen, P. Deák, David M. Glover, Hiroyuki Ohkura

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

We describe a new Drosophila gene, mini spindles (msps) identified in a cytological screen for mitotic mutant. Mutation in msps disrupts the structural integrity of the mitotic spindle, resulting in the formation of one or more small additional spindles in diploid cells. Nucleation of microtubules from centrosomes, metaphase alignment of chromosomes, or the focusing of spindle poles appears much less affected. The msps gene encodes a 227-kD protein with high similarity to the vertebrate microtubule-associated proteins (MAPs), human TOGp and Xenopus XMAP215, and with limited similarity to the Dis1 and STU2 proteins from fission yeast and budding yeast. Consistent with their sequence similarity, Msps protein also associates with microtubules in vitro. In the embryonic division cycles, Msps protein localizes to centrosomal regions at all mitotic stages, and spreads over the spindles during metaphase and anaphase. The absence of centrosomal staining in interphase of the cellularized embryos suggests that the interactions between Msps protein and microtubules or centrosomes may be regulated during the cell cycle.

Original languageEnglish
Pages (from-to)1005-1018
Number of pages14
JournalJournal of Cell Biology
Volume146
Issue number5
DOIs
Publication statusPublished - 1999

Fingerprint

Spindle Apparatus
Microtubule-Associated Proteins
Drosophila
Centrosome
Metaphase
Microtubules
Schizosaccharomyces pombe Proteins
Spindle Poles
Genes
Microtubule Proteins
Anaphase
Proteins
Saccharomycetales
Interphase
Xenopus
Diploidy
Vertebrates
Cell Cycle
Embryonic Structures
Chromosomes

Keywords

  • Cytoskeleton
  • Drosophila
  • Microtubule
  • Mitosis
  • Spindle

ASJC Scopus subject areas

  • Cell Biology

Cite this

mini spindles : A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila. / Cullen, C. Fiona; Deák, P.; Glover, David M.; Ohkura, Hiroyuki.

In: Journal of Cell Biology, Vol. 146, No. 5, 1999, p. 1005-1018.

Research output: Contribution to journalArticle

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