Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis

Begüm Peksel, I. Gombos, Mária Péter, László Vigh, Ádám Tiszlavicz, Mario Brameshuber, G. Balogh, Gerhard J. Schütz, I. Horváth, László Veigh, Z. Török

Research output: Contribution to journalArticle

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Abstract

The current research on cellular heat stress management focuses on the roles of heat shock proteins (HSPs) and the proteostasis network under severe stress conditions. The mild, fever-type stress and the maintenance of membrane homeostasis are less well understood. Herein, we characterized the acute effect of mild, fever-range heat shock on membrane organization, and HSP synthesis and localization in two mammalian cell lines, to delineate the role of membranes in the sensing and adaptation to heat. A multidisciplinary approach combining ultrasensitive fluorescence microscopy and lipidomics revealed the molecular details of novel cellular "eustress", when cells adapt to mild heat by maintaining membrane homeostasis, activating lipid remodeling, and redistributing chaperone proteins. Notably, this leads to acquired thermotolerance in the complete absence of the induction of HSPs. At higher temperatures, additional defense mechanisms are activated, including elevated expression of molecular chaperones, contributing to an extended stress memory and acquired thermotolerance.

Original languageEnglish
Article number15643
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Chinese hamsters
heat shock proteins
protein synthesis
heat tolerance
heat
fever
heat stress
homeostasis
stress management
molecular chaperones
acute effects
cells
fluorescence microscopy
defense mechanisms
cell lines
lipids
temperature
proteins

ASJC Scopus subject areas

  • General

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Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis. / Peksel, Begüm; Gombos, I.; Péter, Mária; Vigh, László; Tiszlavicz, Ádám; Brameshuber, Mario; Balogh, G.; Schütz, Gerhard J.; Horváth, I.; Veigh, László; Török, Z.

In: Scientific Reports, Vol. 7, No. 1, 15643, 01.12.2017.

Research output: Contribution to journalArticle

Peksel, Begüm ; Gombos, I. ; Péter, Mária ; Vigh, László ; Tiszlavicz, Ádám ; Brameshuber, Mario ; Balogh, G. ; Schütz, Gerhard J. ; Horváth, I. ; Veigh, László ; Török, Z. / Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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