Microstructural analysis of the fast gelling freeze-dried sodium hyaluronate

Andrea Krüger-Szabó, Zoltán Aigner, Emese Balogh, István Sebe, Romána Zelkó, István Antal

Research output: Contribution to journalArticle

5 Citations (Scopus)


Although sodium hyaluronate is a very unstable and heat sensitive molecule, it can remain relatively stable during the freeze-drying process. Aqueous sodium hyaluronate (NaHA) gels were prepared and the obtained samples were freeze-dried. The freeze-dried NaHA samples showed fast gelling ability meanwhile preserved their initial viscoelasticity even after reconstitution. The microstructure of gels obtained from raw substance and freeze-dried NaHA samples was characterized with positron annihilation lifetime spectroscopy and X-ray diffraction patterns while their functionality-related macrostructural properties were tested based on their rheological behavior. The presence of phosphate salts improved the formation of ordered supramolecular structure retaining water in the free volume holes of the polymer chains characterized with decreased ortho-positronium lifetime values. This property may be advantageous in the development of a freeze-dried NaHA injection dosage form.

Original languageEnglish
Pages (from-to)12-16
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - Feb 1 2015



  • Fast gelling
  • Freeze-drying
  • Positron annihilation lifetime spectroscopy
  • Sodium hyaluronate (NaHA)
  • Viscoelasticity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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