MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes

Nára Faragó, Liliána Z. Fehér, K. Kitajka, Undurti N. Das, L. Puskás

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background: Polyunsaturated fatty acids (PUFAs) such as -linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells. Results: We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras. Conclusions: Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.

Original languageEnglish
Article number173
JournalLipids in Health and Disease
Volume10
DOIs
Publication statusPublished - 2011

Fingerprint

temozolomide
MicroRNAs
Unsaturated Fatty Acids
Glioma
Apoptosis
Docosahexaenoic Acids
Arachidonic Acid
Genes
alpha-Linolenic Acid
Real-Time Polymerase Chain Reaction
Fatty Acids
Throughput
Messenger RNA

Keywords

  • apoptosis
  • glioblastoma
  • micro RNA
  • PUFA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes. / Faragó, Nára; Fehér, Liliána Z.; Kitajka, K.; Das, Undurti N.; Puskás, L.

In: Lipids in Health and Disease, Vol. 10, 173, 2011.

Research output: Contribution to journalArticle

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AU - Das, Undurti N.

AU - Puskás, L.

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