Abstract
Background: Polyunsaturated fatty acids (PUFAs) such as -linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells. Results: We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras. Conclusions: Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
| Original language | English |
|---|---|
| Article number | 173 |
| Journal | Lipids in Health and Disease |
| Volume | 10 |
| DOIs | |
| Publication status | Published - 2011 |
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Keywords
- apoptosis
- glioblastoma
- micro RNA
- PUFA
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical
- Endocrinology, Diabetes and Metabolism
- Endocrinology
Cite this
MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes. / Faragó, Nára; Fehér, Liliána Z.; Kitajka, K.; Das, Undurti N.; Puskás, L.
In: Lipids in Health and Disease, Vol. 10, 173, 2011.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes
AU - Faragó, Nára
AU - Fehér, Liliána Z.
AU - Kitajka, K.
AU - Das, Undurti N.
AU - Puskás, L.
PY - 2011
Y1 - 2011
N2 - Background: Polyunsaturated fatty acids (PUFAs) such as -linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells. Results: We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras. Conclusions: Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
AB - Background: Polyunsaturated fatty acids (PUFAs) such as -linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells. Results: We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras. Conclusions: Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
KW - apoptosis
KW - glioblastoma
KW - micro RNA
KW - PUFA
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U2 - 10.1186/1476-511X-10-173
DO - 10.1186/1476-511X-10-173
M3 - Article
VL - 10
JO - Lipids in Health and Disease
JF - Lipids in Health and Disease
SN - 1476-511X
M1 - 173
ER -