MicroRNA profile before and after antiviral therapy in liver transplant recipients for hepatitis C virus cirrhosis

Fanni Gelley, Gergely Zadori, B. Nemes, Matteo Fassan, Gabor Lendvai, E. Sárváry, Attila Doros, Zsuzsanna Gerlei, P. Nagy, Z. Schaff, Andras Kiss

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background and Aim: Management of hepatitis C virus (HCV) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin-1, occludin, tetraspanin CD81, scavenger receptor type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV. MicroRNAs (miRs) play role in the regulation of gene expression. We aimed to characterize miR expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on miRs predicted to target HCV receptors. Methods: Twenty-eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MiRs for HCV receptors were selected using target prediction software. Expression levels of miR-21, miR-23a miR-34a, miR-96, miR-99a*, miR-122, miR-125b, miR-181a-2*, miR-194, miR-195, miR-217, miR-221, and miR-224 were determined by reverse transcription-quantitative polymerase chain reaction. Results: miR-99a* and miR-224 expressions were increased in HCV recurrence samples, while miR-21 and miR-194 were decreased in comparison to normal liver tissue. Increased expressions of miR-221, miR-224, and miR-217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of miR-122. Conclusions: Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV-related miR expression profiles, with significant dysregulation of those miRNAs potentially targeting mRNAs of HCV receptors. In particular, miR-194 and miR-21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

MicroRNAs
Hepacivirus
Antiviral Agents
Fibrosis
Liver
Virus Receptors
Therapeutics
Recurrence
Virus Diseases
Transplant Recipients
Claudin-1
Occludin
Scavenger Receptors
Gene Expression Regulation
Viral Load
Liver Transplantation
Reverse Transcription

Keywords

  • HCV
  • HCV receptors
  • Liver transplantation
  • MicroRNA
  • SVR

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

MicroRNA profile before and after antiviral therapy in liver transplant recipients for hepatitis C virus cirrhosis. / Gelley, Fanni; Zadori, Gergely; Nemes, B.; Fassan, Matteo; Lendvai, Gabor; Sárváry, E.; Doros, Attila; Gerlei, Zsuzsanna; Nagy, P.; Schaff, Z.; Kiss, Andras.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 29, No. 1, 01.2014, p. 121-127.

Research output: Contribution to journalArticle

Gelley, Fanni ; Zadori, Gergely ; Nemes, B. ; Fassan, Matteo ; Lendvai, Gabor ; Sárváry, E. ; Doros, Attila ; Gerlei, Zsuzsanna ; Nagy, P. ; Schaff, Z. ; Kiss, Andras. / MicroRNA profile before and after antiviral therapy in liver transplant recipients for hepatitis C virus cirrhosis. In: Journal of Gastroenterology and Hepatology (Australia). 2014 ; Vol. 29, No. 1. pp. 121-127.
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abstract = "Background and Aim: Management of hepatitis C virus (HCV) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin-1, occludin, tetraspanin CD81, scavenger receptor type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV. MicroRNAs (miRs) play role in the regulation of gene expression. We aimed to characterize miR expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on miRs predicted to target HCV receptors. Methods: Twenty-eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MiRs for HCV receptors were selected using target prediction software. Expression levels of miR-21, miR-23a miR-34a, miR-96, miR-99a*, miR-122, miR-125b, miR-181a-2*, miR-194, miR-195, miR-217, miR-221, and miR-224 were determined by reverse transcription-quantitative polymerase chain reaction. Results: miR-99a* and miR-224 expressions were increased in HCV recurrence samples, while miR-21 and miR-194 were decreased in comparison to normal liver tissue. Increased expressions of miR-221, miR-224, and miR-217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of miR-122. Conclusions: Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV-related miR expression profiles, with significant dysregulation of those miRNAs potentially targeting mRNAs of HCV receptors. In particular, miR-194 and miR-21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy.",
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AU - Gelley, Fanni

AU - Zadori, Gergely

AU - Nemes, B.

AU - Fassan, Matteo

AU - Lendvai, Gabor

AU - Sárváry, E.

AU - Doros, Attila

AU - Gerlei, Zsuzsanna

AU - Nagy, P.

AU - Schaff, Z.

AU - Kiss, Andras

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N2 - Background and Aim: Management of hepatitis C virus (HCV) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin-1, occludin, tetraspanin CD81, scavenger receptor type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV. MicroRNAs (miRs) play role in the regulation of gene expression. We aimed to characterize miR expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on miRs predicted to target HCV receptors. Methods: Twenty-eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MiRs for HCV receptors were selected using target prediction software. Expression levels of miR-21, miR-23a miR-34a, miR-96, miR-99a*, miR-122, miR-125b, miR-181a-2*, miR-194, miR-195, miR-217, miR-221, and miR-224 were determined by reverse transcription-quantitative polymerase chain reaction. Results: miR-99a* and miR-224 expressions were increased in HCV recurrence samples, while miR-21 and miR-194 were decreased in comparison to normal liver tissue. Increased expressions of miR-221, miR-224, and miR-217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of miR-122. Conclusions: Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV-related miR expression profiles, with significant dysregulation of those miRNAs potentially targeting mRNAs of HCV receptors. In particular, miR-194 and miR-21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy.

AB - Background and Aim: Management of hepatitis C virus (HCV) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin-1, occludin, tetraspanin CD81, scavenger receptor type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV. MicroRNAs (miRs) play role in the regulation of gene expression. We aimed to characterize miR expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on miRs predicted to target HCV receptors. Methods: Twenty-eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MiRs for HCV receptors were selected using target prediction software. Expression levels of miR-21, miR-23a miR-34a, miR-96, miR-99a*, miR-122, miR-125b, miR-181a-2*, miR-194, miR-195, miR-217, miR-221, and miR-224 were determined by reverse transcription-quantitative polymerase chain reaction. Results: miR-99a* and miR-224 expressions were increased in HCV recurrence samples, while miR-21 and miR-194 were decreased in comparison to normal liver tissue. Increased expressions of miR-221, miR-224, and miR-217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of miR-122. Conclusions: Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV-related miR expression profiles, with significant dysregulation of those miRNAs potentially targeting mRNAs of HCV receptors. In particular, miR-194 and miR-21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy.

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KW - HCV receptors

KW - Liver transplantation

KW - MicroRNA

KW - SVR

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