MicroRNA-132 targets HB-EGF upon IgE-mediated activation in murine and human mast cells

Viktor Molnár, Barbara É Rsek, Zoltán Wiener, Zsófia Tömböl, Péter M. Szabó, Péter Igaz, András Falus

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

MicroRNAs provide an additional layer in the regulation of gene expression acting as repressors with several targets at the posttranscriptional level. This study describes microRNA expression patterns during differentiation and activation of mast cells. The expression levels of 567 different mouse miRNAs were compared by microarray between c-Kit? committed progenitors, mucosal mast cells, resting and IgE-crosslinked BMMCs in vitro. The strongest upregulation of miR-132 upon IgEmediated activation was validated in human cord bloodderived mast cells as well. HB-EGF growth factor also upregulated upon activation and was ranked high by more prediction algorithms. Co-transfection of miR-132 mimicking precursor and the 30UTR of human Hbegfcontaining luciferase vector proves that the predicted binding site is functional. In line with this, neutralization of miR-132 by anti-miR inhibitor leads to sustained production of HB-EGF protein in activated mast cells. Our data provide a novel example for negative regulation of a growth factor by an upregulated miRNA.

Original languageEnglish
Pages (from-to)793-808
Number of pages16
JournalCellular and Molecular Life Sciences
Volume69
Issue number5
DOIs
Publication statusPublished - Mar 1 2012

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Keywords

  • Activation
  • Differentiation
  • HB-EGF
  • IgE
  • Mast cells
  • MicroRNAs

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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