Micropropagation of lemon balm

Annamária Mészáros, Andrea Bellon, Éva Pintér, Gábor Horváth

Research output: Contribution to journalArticle

9 Citations (Scopus)


Traditional propagation of lemon balm (Melissa officinalis L.) is inefficient for establishing a good quality clonal population. Results of the presented experiments outline an effective method for micropropagation of this species. Following culture initiation from shoots of field-grown plants on growth regulator free Murashige-Skoog medium, rapid shoot multiplication with only rudimentary root formation could be achieved on media containing various concentrations of indole-3-acetic acid and 6-benzyladenine. The combination of 5.71 μM indole-3-acetic acid and 6.66 μM 6-benzyladenine resulted in the best multiplication. Transfer of propagules to media containing indole-3-acetic acid and kinetin did not result in shoot proliferation; however, single plantlets grown on media containing 5.71 μM indole-3-acetic acid and 13.9 μM kinetin developed more compact shoots and stronger roots than the control plants and were suitable for acclimatization with an efficiency over 95%.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Issue number2
Publication statusPublished - Dec 1 1999


  • Growth regulators
  • Melissa officinalis
  • Organogenesis

ASJC Scopus subject areas

  • Horticulture

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