Microplasma induced cell morphological changes and apoptosis of Ex vivo cultured human anterior lens epithelial cells relevance to capsular opacification

Nina Recek, Sofija Andjelić, Nataša Hojnik, Gregor Filipič, Sasa Lazović, Alenka Vesel, Gregor Primc, Miran Mozetič, Marko Hawlina, G. Petrovski, Uros Cvelbar

Research output: Contribution to journalArticle

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Abstract

Inducing selective or targeted cell apoptosis without affecting large number of neighbouring cells remains a challenge. A plausible method for treatment of posterior capsular opacification (PCO) due to remaining lens epithelial cells (LECs) by reactive chemistry induced by localized single electrode microplasma discharge at top of a needle-like glass electrode with spot size ∼3 μm is hereby presented. The focused and highly-localized atmospheric pressure microplasma jet with electrode discharge could induce a dose-dependent apoptosis in selected and targeted individual LECs, which could be confirmed by real-time monitoring of the morphological and structural changes at cellular level. Direct cell treatment with microplasma inside the medium appeared more effective in inducing apoptosis (caspase 8 positivity and DNA fragmentation) at a highly targeted cell level compared to treatment on top of the medium (indirect treatment). Our results show that single cell specific micropipette plasma can be used to selectively induce demise in LECs which remain in the capsular bag after cataract surgery and thus prevent their migration (CXCR4 positivity) to the posterior lens capsule and PCO formation.

Original languageEnglish
Article numbere0165883
JournalPLoS One
Volume11
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Lens
Lenses
epithelial cells
apoptosis
Posterior Capsule of the Lens
Epithelial Cells
Apoptosis
Electrodes
electrodes
cells
Atmospheric Pressure
Caspase 8
DNA Fragmentation
caspase-8
Needles
Cataract
Surgery
cataract
Atmospheric pressure
Capsules

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Microplasma induced cell morphological changes and apoptosis of Ex vivo cultured human anterior lens epithelial cells relevance to capsular opacification. / Recek, Nina; Andjelić, Sofija; Hojnik, Nataša; Filipič, Gregor; Lazović, Sasa; Vesel, Alenka; Primc, Gregor; Mozetič, Miran; Hawlina, Marko; Petrovski, G.; Cvelbar, Uros.

In: PLoS One, Vol. 11, No. 11, e0165883, 01.11.2016.

Research output: Contribution to journalArticle

Recek, N, Andjelić, S, Hojnik, N, Filipič, G, Lazović, S, Vesel, A, Primc, G, Mozetič, M, Hawlina, M, Petrovski, G & Cvelbar, U 2016, 'Microplasma induced cell morphological changes and apoptosis of Ex vivo cultured human anterior lens epithelial cells relevance to capsular opacification', PLoS One, vol. 11, no. 11, e0165883. https://doi.org/10.1371/journal.pone.0165883
Recek, Nina ; Andjelić, Sofija ; Hojnik, Nataša ; Filipič, Gregor ; Lazović, Sasa ; Vesel, Alenka ; Primc, Gregor ; Mozetič, Miran ; Hawlina, Marko ; Petrovski, G. ; Cvelbar, Uros. / Microplasma induced cell morphological changes and apoptosis of Ex vivo cultured human anterior lens epithelial cells relevance to capsular opacification. In: PLoS One. 2016 ; Vol. 11, No. 11.
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