In vitro fertilization and related assisted reproductive techniques opened a new prospect in the medical treatment of human infertility during the last two decades. Since their invention micromanipulators were frequently used in several fields of biological research. Several micromanipulation techniques gained ground in assisted reproduction during the last 10 years. In this review the working principle of micromanipulators is described as followed by an overview of micromanipulation procedures applied for improving the results of the in vitro fertilization treatment: different procedures for opening the zona pellucida of human oocytes and embryos, subzonal sperm injection and intracytoplasmic sperm injection. The possibility of the micromanipulative correction of abnormal fertilization is analyzed and the application of micromanipulation for the preimplantation genetic diagnosis is discussed.
|Number of pages||10|
|Journal||Lege Artis Medicinae|
|Publication status||Published - Dec 1 1997|
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