Micromanipulation of the human oocyte

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Intracytoplasmic sperm injection (ICSI) provides an excellent outcome in a consistent manner, and is therefore used worldwide as a routine procedure. Since its introduction, few modifications have been made to its methodology. Recently, a combination of ICSI with micro-hole drilling by laser (LA-ICSI) of the zona pellucida appeared to decrease oocyte degeneration rates and to improve embryo quality and implantation. Cytoplasmic transfer is a more recently introduced procedure where the objective is to improve the quality of patients' oocytes by transferring cytoplasm from a good quality donor oocyte, in cases where it is assumed that cytoplasm is compromised. Nuclear transfer, involving exchange of nuclei between donor and receptor oocytes, is still an experimental procedure, the objective being similar to cytoplasmic transfer in improving oocyte/embryo quality. A nuclear transfer procedure involving somatic cells for reproductive purposes should not be used in humans, for ethical and technical considerations. On the other hand, nuclear transfer for therapeutic purposes to obtain stem cells may be considered in respect of its unique potential in medicine. Finally, the most recently emerged new concept under investigation is the haploidization of somatic cells for the purpose of creating artificial gametes.

Original languageEnglish
Pages (from-to)634-640
Number of pages7
JournalReproductive BioMedicine Online
Volume7
Issue number6
Publication statusPublished - Dec 2003

Fingerprint

Micromanipulation
Oocytes
Intracytoplasmic Sperm Injections
Cytoplasm
Tissue Donors
Zona Pellucida
Germ Cells
Lasers
Stem Cells
Embryonic Structures
Medicine

Keywords

  • Embryo donation
  • Ethics
  • Haemophilia
  • ICSI
  • Oocyte donation
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Micromanipulation of the human oocyte. / Nagy, P.

In: Reproductive BioMedicine Online, Vol. 7, No. 6, 12.2003, p. 634-640.

Research output: Contribution to journalArticle

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