Microheterogeneity characterization of a trichorzianine-a mixture from Trichoderma harzianum

Gabriella Pócsfalvi, Felice Scala, Matteo Lorito, Alberto Ritieni, Giacomino Randazzo, Pasquale Ferranti, K. Vékey, Antonio Malorni

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The microheterogeneity of trichorzianine-A (TA) from Trichoderma harzianum was studied using single-stage mass spectrometry and high-energy collision-induced dissociation (CID) tandem mass spectrometry. Based on the electrospray ionization and the liquid secondary ion single-state mass spectra, the main components with eight different molecular masses were identified in the mixture. Five contain phenylalaninol and three a tryptophanol C-terminal amino alcohol residue. The tandem mass spectra taken on the protonated molecular ion of these components (MH+) show that most of them contain one main and one or more minor sequence analogues. For the microheterogeneity characterization of this mixture, a previously described method was used based on high-energy CID studies of the different MH+, y7, b12 ions and their satellite ions with ± 14 and ± 28 mass number differences. In addition to the nine known sequence variants, five new trichorzianines were sequenced from the mixture. It was found, furthermore, that amino acid exchange can occur not only at positions 5, 14, 16 and 19 but also at positions 2, 3, 4, 7, 8 and 9.

Original languageEnglish
Pages (from-to)154-163
Number of pages10
JournalJournal of Mass Spectrometry
Volume33
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Trichoderma
Ions
mass spectra
mass spectroscopy
dissociation
Mass spectrometry
Peptaibols
ions
collisions
Amino alcohols
Amino Alcohols
molecular ions
Electrospray ionization
amino acids
Molecular mass
alcohols
Tandem Mass Spectrometry
analogs
Amino acids
ionization

Keywords

  • High-energy collision-induced dissociation
  • Peptaibols
  • Peptide sequencing
  • Tandem mass spectrometry
  • Trichorzianines

ASJC Scopus subject areas

  • Biophysics
  • Organic Chemistry
  • Spectroscopy

Cite this

Microheterogeneity characterization of a trichorzianine-a mixture from Trichoderma harzianum. / Pócsfalvi, Gabriella; Scala, Felice; Lorito, Matteo; Ritieni, Alberto; Randazzo, Giacomino; Ferranti, Pasquale; Vékey, K.; Malorni, Antonio.

In: Journal of Mass Spectrometry, Vol. 33, No. 2, 1998, p. 154-163.

Research output: Contribution to journalArticle

Pócsfalvi, Gabriella ; Scala, Felice ; Lorito, Matteo ; Ritieni, Alberto ; Randazzo, Giacomino ; Ferranti, Pasquale ; Vékey, K. ; Malorni, Antonio. / Microheterogeneity characterization of a trichorzianine-a mixture from Trichoderma harzianum. In: Journal of Mass Spectrometry. 1998 ; Vol. 33, No. 2. pp. 154-163.
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