Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites

Krisztina Nagy, Orsolya Sipos, Sándor Valkai, Éva Gombai, Orsolya Hodula, Ádám Kerényi, P. Ormos, P. Galajda

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Quorum sensing and chemotaxis both affect bacterial behavior on the population level. Chemotaxis shapes the spatial distribution of cells, while quorum sensing realizes a cell-density dependent gene regulation. An interesting question is if these mechanisms interact on some level: Does quorum sensing, a density dependent process, affect cell density itself via chemotaxis? Since quorum sensing often spans across species, such a feedback mechanism may also exist between multiple species. We constructed a microfluidic platform to study these questions. A flow-free, stable linear chemical gradient is formed in our device within a few minutes that makes it suitable for sensitive testing of chemoeffectors: we showed that the amino acid lysine is a weak chemoattractant for Escherichia coli, while arginine is neutral. We studied the effect of quorum sensing signal molecules of Pseudomonas aeruginosa on E. coli chemotaxis. Our results show that N-(3-oxododecanoyl)-homoserine lactone (oxo-C12-HSL) and N-(butryl)-homoserine lactone (C4-HSL) are attractants. Furthermore, we tested the chemoeffector potential of pyocyanin and pyoverdine, secondary metabolites under a quorum sensing control. Pyocyanin is proved to be a weak attractant while pyoverdine are repellent. We demonstrated the usability of the device in co-culturing experiments, where we showed that various factors released by P. aeruginosa affect the dynamic spatial rearrangement of a neighboring E. coli population, while surface adhesion of the cells is also modulated.

Original languageEnglish
Article number044105
JournalBiomicrofluidics
Volume9
Issue number4
DOIs
Publication statusPublished - 2015

Fingerprint

Quorum Sensing
Microfluidics
metabolites
Pyocyanine
Metabolites
Escherichia
Escherichia coli
amino acids
Amino acids
Chemotaxis
Amino Acids
Molecules
molecules
Arginine
Chemotactic Factors
cells
Gene expression
Pseudomonas aeruginosa
Spatial distribution
Lysine

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites. / Nagy, Krisztina; Sipos, Orsolya; Valkai, Sándor; Gombai, Éva; Hodula, Orsolya; Kerényi, Ádám; Ormos, P.; Galajda, P.

In: Biomicrofluidics, Vol. 9, No. 4, 044105, 2015.

Research output: Contribution to journalArticle

Nagy, Krisztina ; Sipos, Orsolya ; Valkai, Sándor ; Gombai, Éva ; Hodula, Orsolya ; Kerényi, Ádám ; Ormos, P. ; Galajda, P. / Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites. In: Biomicrofluidics. 2015 ; Vol. 9, No. 4.
@article{3e6b20fca36f4527a0e2f5a07f5167ca,
title = "Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites",
abstract = "Quorum sensing and chemotaxis both affect bacterial behavior on the population level. Chemotaxis shapes the spatial distribution of cells, while quorum sensing realizes a cell-density dependent gene regulation. An interesting question is if these mechanisms interact on some level: Does quorum sensing, a density dependent process, affect cell density itself via chemotaxis? Since quorum sensing often spans across species, such a feedback mechanism may also exist between multiple species. We constructed a microfluidic platform to study these questions. A flow-free, stable linear chemical gradient is formed in our device within a few minutes that makes it suitable for sensitive testing of chemoeffectors: we showed that the amino acid lysine is a weak chemoattractant for Escherichia coli, while arginine is neutral. We studied the effect of quorum sensing signal molecules of Pseudomonas aeruginosa on E. coli chemotaxis. Our results show that N-(3-oxododecanoyl)-homoserine lactone (oxo-C12-HSL) and N-(butryl)-homoserine lactone (C4-HSL) are attractants. Furthermore, we tested the chemoeffector potential of pyocyanin and pyoverdine, secondary metabolites under a quorum sensing control. Pyocyanin is proved to be a weak attractant while pyoverdine are repellent. We demonstrated the usability of the device in co-culturing experiments, where we showed that various factors released by P. aeruginosa affect the dynamic spatial rearrangement of a neighboring E. coli population, while surface adhesion of the cells is also modulated.",
author = "Krisztina Nagy and Orsolya Sipos and S{\'a}ndor Valkai and {\'E}va Gombai and Orsolya Hodula and {\'A}d{\'a}m Ker{\'e}nyi and P. Ormos and P. Galajda",
year = "2015",
doi = "10.1063/1.4926981",
language = "English",
volume = "9",
journal = "Biomicrofluidics",
issn = "1932-1058",
publisher = "American Institute of Physics Publising LLC",
number = "4",

}

TY - JOUR

T1 - Microfluidic study of the chemotactic response of Escherichia coli to amino acids, signaling molecules and secondary metabolites

AU - Nagy, Krisztina

AU - Sipos, Orsolya

AU - Valkai, Sándor

AU - Gombai, Éva

AU - Hodula, Orsolya

AU - Kerényi, Ádám

AU - Ormos, P.

AU - Galajda, P.

PY - 2015

Y1 - 2015

N2 - Quorum sensing and chemotaxis both affect bacterial behavior on the population level. Chemotaxis shapes the spatial distribution of cells, while quorum sensing realizes a cell-density dependent gene regulation. An interesting question is if these mechanisms interact on some level: Does quorum sensing, a density dependent process, affect cell density itself via chemotaxis? Since quorum sensing often spans across species, such a feedback mechanism may also exist between multiple species. We constructed a microfluidic platform to study these questions. A flow-free, stable linear chemical gradient is formed in our device within a few minutes that makes it suitable for sensitive testing of chemoeffectors: we showed that the amino acid lysine is a weak chemoattractant for Escherichia coli, while arginine is neutral. We studied the effect of quorum sensing signal molecules of Pseudomonas aeruginosa on E. coli chemotaxis. Our results show that N-(3-oxododecanoyl)-homoserine lactone (oxo-C12-HSL) and N-(butryl)-homoserine lactone (C4-HSL) are attractants. Furthermore, we tested the chemoeffector potential of pyocyanin and pyoverdine, secondary metabolites under a quorum sensing control. Pyocyanin is proved to be a weak attractant while pyoverdine are repellent. We demonstrated the usability of the device in co-culturing experiments, where we showed that various factors released by P. aeruginosa affect the dynamic spatial rearrangement of a neighboring E. coli population, while surface adhesion of the cells is also modulated.

AB - Quorum sensing and chemotaxis both affect bacterial behavior on the population level. Chemotaxis shapes the spatial distribution of cells, while quorum sensing realizes a cell-density dependent gene regulation. An interesting question is if these mechanisms interact on some level: Does quorum sensing, a density dependent process, affect cell density itself via chemotaxis? Since quorum sensing often spans across species, such a feedback mechanism may also exist between multiple species. We constructed a microfluidic platform to study these questions. A flow-free, stable linear chemical gradient is formed in our device within a few minutes that makes it suitable for sensitive testing of chemoeffectors: we showed that the amino acid lysine is a weak chemoattractant for Escherichia coli, while arginine is neutral. We studied the effect of quorum sensing signal molecules of Pseudomonas aeruginosa on E. coli chemotaxis. Our results show that N-(3-oxododecanoyl)-homoserine lactone (oxo-C12-HSL) and N-(butryl)-homoserine lactone (C4-HSL) are attractants. Furthermore, we tested the chemoeffector potential of pyocyanin and pyoverdine, secondary metabolites under a quorum sensing control. Pyocyanin is proved to be a weak attractant while pyoverdine are repellent. We demonstrated the usability of the device in co-culturing experiments, where we showed that various factors released by P. aeruginosa affect the dynamic spatial rearrangement of a neighboring E. coli population, while surface adhesion of the cells is also modulated.

UR - http://www.scopus.com/inward/record.url?scp=84937243269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937243269&partnerID=8YFLogxK

U2 - 10.1063/1.4926981

DO - 10.1063/1.4926981

M3 - Article

AN - SCOPUS:84937243269

VL - 9

JO - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 4

M1 - 044105

ER -