Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles - An efficient and flexible novel tool for enzyme catalyzed biotransformations

Ferenc Ender, Diána Weiser, Botond Nagy, Csaba László Bencze, Csaba Paizs, Péter Pálovics, L. Poppe

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Biotransformation of L-phenylalanine (L-1a) and five unnatural substrates (rac-1b-f) by phenylalanine ammonia-lyase (PAL) was investigated in a novel microfluidic device (Magne-Chip) that comprises microliter volume reaction cells filled with PAL-coated magnetic nanoparticles (MNPs). Experiments proved the excellent reproducibility of enzymecatalyzed biotransformation in the chip and the excellent reusability of the enzyme layer during 14 h continuous measurement (>98% over 7 repetitive measurements with L-1a). The platform also enabled fully automatic multiparameter measurements with a single biocatalyst loading of about 1 mg PAL-MNP. Computational fluid dynamics (CFD) calculations were used to study the flow field in the chambers and the effect of unintended bubble formation. Optimal flow rate for L-1a reaction and specific activities for rac-1b-f under these conditions were determined.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalJournal of Flow Chemistry
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Phenylalanine Ammonia-Lyase
Microfluidics
Ammonia
Enzymes
Nanoparticles
Bubble formation
Biocatalysts
Reusability
Phenylalanine
Flow fields
Computational fluid dynamics
Flow rate
Substrates
Biotransformation
Experiments

Keywords

  • Continuous-flow biotransformation
  • Magnetic chip reactor
  • Magnetic nanoparticle
  • Phenylalanine ammonia-lyase
  • Unnatural amino acid

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Fluid Flow and Transfer Processes
  • Organic Chemistry

Cite this

Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles - An efficient and flexible novel tool for enzyme catalyzed biotransformations. / Ender, Ferenc; Weiser, Diána; Nagy, Botond; Bencze, Csaba László; Paizs, Csaba; Pálovics, Péter; Poppe, L.

In: Journal of Flow Chemistry, Vol. 6, No. 1, 01.03.2016, p. 43-52.

Research output: Contribution to journalArticle

Ender, Ferenc ; Weiser, Diána ; Nagy, Botond ; Bencze, Csaba László ; Paizs, Csaba ; Pálovics, Péter ; Poppe, L. / Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles - An efficient and flexible novel tool for enzyme catalyzed biotransformations. In: Journal of Flow Chemistry. 2016 ; Vol. 6, No. 1. pp. 43-52.
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AU - Pálovics, Péter

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