Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles - An efficient and flexible novel tool for enzyme catalyzed biotransformations

Ferenc Ender, Diána Weiser, Botond Nagy, Csaba László Bencze, Csaba Paizs, Péter Pálovics, László Poppe

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Biotransformation of L-phenylalanine (L-1a) and five unnatural substrates (rac-1b-f) by phenylalanine ammonia-lyase (PAL) was investigated in a novel microfluidic device (Magne-Chip) that comprises microliter volume reaction cells filled with PAL-coated magnetic nanoparticles (MNPs). Experiments proved the excellent reproducibility of enzymecatalyzed biotransformation in the chip and the excellent reusability of the enzyme layer during 14 h continuous measurement (>98% over 7 repetitive measurements with L-1a). The platform also enabled fully automatic multiparameter measurements with a single biocatalyst loading of about 1 mg PAL-MNP. Computational fluid dynamics (CFD) calculations were used to study the flow field in the chambers and the effect of unintended bubble formation. Optimal flow rate for L-1a reaction and specific activities for rac-1b-f under these conditions were determined.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalJournal of Flow Chemistry
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 2016

Keywords

  • Continuous-flow biotransformation
  • Magnetic chip reactor
  • Magnetic nanoparticle
  • Phenylalanine ammonia-lyase
  • Unnatural amino acid

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Fluid Flow and Transfer Processes
  • Organic Chemistry

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