Microfluidic channels laser-cut in thin double-sided tapes: Cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips

Daniel Patko, Zsolt Mártonfalvi, Boglarka Kovacs, F. Vonderviszt, M. Kellermayer, R. Horváth

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A simple, reliable and cost-effective fluidic channel, fabricated by using double-sided pressure-sensitive tapes, is demonstrated here. A laser-cutting method is applied to engrave structures in sheets of the tapes. After peeling off the tape liners, the structures could be easily integrated at room temperature with label-free optical waveguide biochips without further modifications or additional processing steps. It is shown that the well-defined and controllable height of the channels is advantageous for stopped-flow measurements of analyte binding. The easy fabrication of a fully transparent integrated sensor unit - tape cuvette system is also demonstrated for parallel microscopic investigations. The transparent unit was used to on-line monitor the surface adhesion of Salmonella cells on poly-l-lysine-coated biochip surfaces, followed by the straightforward microscopic visualization of the adhered bacterial cells. The material of the double sided tape is stable in aqueous solutions. Furthermore, its material is biocompatible, making it ideal for biological applications. Excellent, stable and reversible bonding of the microstructured tapes to biocompatible plastic and glass is also demonstrated. The simplicity of the fabrication at ambient temperatures makes the developed processes appealing for lab-on-a-chip applications, particularly if the bonded biochips are precious.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume196
DOIs
Publication statusPublished - Jun 2014

Fingerprint

Biochips
fluidics
Fluidics
Microfluidics
Tapes
tapes
costs
Lasers
lasers
Costs
salmonella
laser cutting
Fabrication
Lab-on-a-chip
fabrication
Salmonella
peeling
Peeling
lysine
flow measurement

Keywords

  • Bacteria
  • Double sided tape
  • Label-free biochip
  • Laser cutter
  • Microfluidics
  • Pressure sensitive tape

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

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abstract = "A simple, reliable and cost-effective fluidic channel, fabricated by using double-sided pressure-sensitive tapes, is demonstrated here. A laser-cutting method is applied to engrave structures in sheets of the tapes. After peeling off the tape liners, the structures could be easily integrated at room temperature with label-free optical waveguide biochips without further modifications or additional processing steps. It is shown that the well-defined and controllable height of the channels is advantageous for stopped-flow measurements of analyte binding. The easy fabrication of a fully transparent integrated sensor unit - tape cuvette system is also demonstrated for parallel microscopic investigations. The transparent unit was used to on-line monitor the surface adhesion of Salmonella cells on poly-l-lysine-coated biochip surfaces, followed by the straightforward microscopic visualization of the adhered bacterial cells. The material of the double sided tape is stable in aqueous solutions. Furthermore, its material is biocompatible, making it ideal for biological applications. Excellent, stable and reversible bonding of the microstructured tapes to biocompatible plastic and glass is also demonstrated. The simplicity of the fabrication at ambient temperatures makes the developed processes appealing for lab-on-a-chip applications, particularly if the bonded biochips are precious.",
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AU - Patko, Daniel

AU - Mártonfalvi, Zsolt

AU - Kovacs, Boglarka

AU - Vonderviszt, F.

AU - Kellermayer, M.

AU - Horváth, R.

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