Microfabricated amperometric cells for multicomponent analysis

Ying Liu, R. Gyurcsányi, Gyula Jágerszki, John D. Denuzzio, Erno Lindner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Towards the development of multianalyte electrochemical immunoassays three individually addressable microelectrode array (MEA) type working electrodes and a reference electrode were integrated into a 4 mL volume, planar electrochemical cell. To model the simultaneous determination of multiple antigens in the cell with enzyme linked immunosorbent assays (ELISAs) glucose oxidase (GOx), alkaline phosphatase (ALP), and b-galactosidase (b-GAL) were immobilized site specifically onto the individual MEA surfaces and the biocatalitic activity of these surface confined enzymes were evaluated by measuring the products of the enzyme catalyzed reactions directly on the gold MEA surfaces by chronoamperometry or by imaging the enzyme patterned microelectrode array surfaces by Scanning Electrochemical Microscopy (SECM). ALP and b-GAL were selected as model enzymes because they are the most commonly used enzymes labels in ELISAs. In these measurements glucose, ascorbic acid phosphate (AAP), and p-aminophenyl-b-d-galactopyranoside (PAPG) served as enzyme substrates, respectively. The electrochemical surface area of the gold MEAs did not change during the multistep immobilization process. All enzyme modified MEAs presented selective and proportional responses to their substrates and the response characteristics of the enzyme modified sensors were identical in separate and simultaneous calibration protocols, i.e., there was no crosscontamination between the closely placed MEAs. The SECM images of the enzyme patterned MEA surfaces suggest that nonspecific adsorption is negligible on the insulating polyimide surface of the MEA separating the individual microelectrode sites.

Original languageEnglish
Pages (from-to)1944-1954
Number of pages11
JournalElectroanalysis
Volume21
Issue number17-18
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Enzymes
Microelectrodes
Galactosidases
Immunosorbents
Phosphatases
Gold
Alkaline Phosphatase
Assays
Microscopic examination
Scanning
Glucose Oxidase
Chronoamperometry
Electrodes
Glucose oxidase
Electrochemical cells
Ascorbic acid
Substrates
Antigens
Galactose
Polyimides

Keywords

  • Biocatalysis
  • Chronoamperometry
  • Enzymes
  • Immobilized enzymes
  • Microelectrode array
  • Multicomponent analysis
  • SECM imaging

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Liu, Y., Gyurcsányi, R., Jágerszki, G., Denuzzio, J. D., & Lindner, E. (2009). Microfabricated amperometric cells for multicomponent analysis. Electroanalysis, 21(17-18), 1944-1954. https://doi.org/10.1002/elan.200904614

Microfabricated amperometric cells for multicomponent analysis. / Liu, Ying; Gyurcsányi, R.; Jágerszki, Gyula; Denuzzio, John D.; Lindner, Erno.

In: Electroanalysis, Vol. 21, No. 17-18, 09.2009, p. 1944-1954.

Research output: Contribution to journalArticle

Liu, Y, Gyurcsányi, R, Jágerszki, G, Denuzzio, JD & Lindner, E 2009, 'Microfabricated amperometric cells for multicomponent analysis', Electroanalysis, vol. 21, no. 17-18, pp. 1944-1954. https://doi.org/10.1002/elan.200904614
Liu, Ying ; Gyurcsányi, R. ; Jágerszki, Gyula ; Denuzzio, John D. ; Lindner, Erno. / Microfabricated amperometric cells for multicomponent analysis. In: Electroanalysis. 2009 ; Vol. 21, No. 17-18. pp. 1944-1954.
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