Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Maria Bosserdt, Júlia Erdossy, Gergely Lautner, Julia Witt, Katja Köhler, Nenad Gajovic-Eichelmann, Aysu Yarman, Gunther Wittstock, Frieder W. Scheller, R. Gyurcsányi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500. μm diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalBiosensors and Bioelectronics
Volume73
DOIs
Publication statusPublished - Nov 5 2015

Fingerprint

Protein Array Analysis
Microarrays
Polymers
Gold
Proteins
Metrorrhagia
Monomers
Imaging techniques
Scopoletin
Ferritins
Labels
Electrodes
Throughput
Kinetics
Chemical analysis

Keywords

  • Electropolymerization
  • Electrospotting
  • Ferritin
  • Molecularly imprinted polymers
  • Scopoletin
  • Surface imprinting

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition. / Bosserdt, Maria; Erdossy, Júlia; Lautner, Gergely; Witt, Julia; Köhler, Katja; Gajovic-Eichelmann, Nenad; Yarman, Aysu; Wittstock, Gunther; Scheller, Frieder W.; Gyurcsányi, R.

In: Biosensors and Bioelectronics, Vol. 73, 05.11.2015, p. 123-129.

Research output: Contribution to journalArticle

Bosserdt, M, Erdossy, J, Lautner, G, Witt, J, Köhler, K, Gajovic-Eichelmann, N, Yarman, A, Wittstock, G, Scheller, FW & Gyurcsányi, R 2015, 'Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition', Biosensors and Bioelectronics, vol. 73, pp. 123-129. https://doi.org/10.1016/j.bios.2015.05.049
Bosserdt, Maria ; Erdossy, Júlia ; Lautner, Gergely ; Witt, Julia ; Köhler, Katja ; Gajovic-Eichelmann, Nenad ; Yarman, Aysu ; Wittstock, Gunther ; Scheller, Frieder W. ; Gyurcsányi, R. / Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition. In: Biosensors and Bioelectronics. 2015 ; Vol. 73. pp. 123-129.
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