Microcystin-LR induces abnormal root development by altering microtubule organization in tissue-cultured common reed (Phragmites australis) plantlets

Csaba Máthé, Dániel Beyer, Ferenc Erdodi, Zoltán Serfozo, Lóránt Székvölgyi, Gábor Vasas, Márta M-Hamvas, Katalin Jámbrik, Sándor Gonda, Andrea Kiss, Zsuzsa M. Szigeti, Gyula Surányi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Microcystin-LR (MC-LR) is a heptapeptide cyanotoxin, known to be a potent inhibitor of type 1 and 2A protein phosphatases in eukaryotes. Our aim was to investigate the effect of MC-LR on the organization of microtubules and mitotic chromatin in relation to its possible effects on cell and whole organ morphology in roots of common reed (Phragmites australis). P. australis is a widespread freshwater and brackish water aquatic macrophyte, frequently exposed to phytotoxins in eutrophic waters. Reed plantlets regenerated from embryogenic calli were treated with 0.001-40 μg ml-1 (0.001-40.2 μM) MC-LR for 2-20 days. At 0.5 μg ml-1 MC-LR and at higher cyanotoxin concentrations, the inhibition of protein phosphatase activity by MC-LR induced alterations in reed root growth and morphology, including abnormal lateral root development and the radial swelling of cells in the elongation zone of primary and lateral roots. Both short-term (2-5 days) and long-term (10-20 days) of cyanotoxin treatment induced microtubule disruption in meristems and in the elongation and differentiation zones. Microtubule disruption was accompanied by root cell shape alteration. At concentrations of 0.5-5 μg ml-1, MC-LR increased mitotic index at long-term exposure and induced the increase of the percentage of meristematic cells in prophase as well as telophase and cytokinesis of late mitosis. High cyanotoxin concentrations (10-40 μg ml-1) inhibited mitosis at as short as 2 days of exposure. The alteration of microtubule organization was observed in mitotic cells at all exposure periods studied, at cyanotoxin concentrations of 0.5-40 μg ml-1. MC-LR induced spindle anomalies at the metaphase-anaphase transition, the formation of asymmetric anaphase spindles and abnormal sister chromatid separation. This paper reports for the first time that MC-LR induces cytoskeletal changes that lead to alterations of root architecture and development in common reed and generally, in plant cells. The MC-LR induced alterations in cells of an ecologically important aquatic macrophyte can reveal the importance of the effects of a cyanobacterial toxin in aquatic ecosystems.

Original languageEnglish
Pages (from-to)122-130
Number of pages9
JournalAquatic Toxicology
Volume92
Issue number3
DOIs
Publication statusPublished - May 5 2009

Fingerprint

microcystin-LR
Phragmites australis
Microtubules
microtubules
cyanobacterial toxins
plantlets
macrophyte
phosphatase
phosphoprotein phosphatase
root architecture
Anaphase
anaphase
protein
cells
Mitosis
eukaryote
mitosis
brackish water
aquatic ecosystem
swelling

Keywords

  • Microcystin-LR
  • Microtubules
  • Mitotic alterations
  • Phragmites australis
  • Protein phosphatase

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Microcystin-LR induces abnormal root development by altering microtubule organization in tissue-cultured common reed (Phragmites australis) plantlets. / Máthé, Csaba; Beyer, Dániel; Erdodi, Ferenc; Serfozo, Zoltán; Székvölgyi, Lóránt; Vasas, Gábor; M-Hamvas, Márta; Jámbrik, Katalin; Gonda, Sándor; Kiss, Andrea; Szigeti, Zsuzsa M.; Surányi, Gyula.

In: Aquatic Toxicology, Vol. 92, No. 3, 05.05.2009, p. 122-130.

Research output: Contribution to journalArticle

Máthé, Csaba ; Beyer, Dániel ; Erdodi, Ferenc ; Serfozo, Zoltán ; Székvölgyi, Lóránt ; Vasas, Gábor ; M-Hamvas, Márta ; Jámbrik, Katalin ; Gonda, Sándor ; Kiss, Andrea ; Szigeti, Zsuzsa M. ; Surányi, Gyula. / Microcystin-LR induces abnormal root development by altering microtubule organization in tissue-cultured common reed (Phragmites australis) plantlets. In: Aquatic Toxicology. 2009 ; Vol. 92, No. 3. pp. 122-130.
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AU - Serfozo, Zoltán

AU - Székvölgyi, Lóránt

AU - Vasas, Gábor

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AU - Jámbrik, Katalin

AU - Gonda, Sándor

AU - Kiss, Andrea

AU - Szigeti, Zsuzsa M.

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