Microcirculation and intracellular free calcium changes in the pregnant uterine muscle

Z. Ruttner, T. Ivanics, F. Kozma, D. W. Slaaf, R. S. Reneman, L. Ligeti, A. Tóth

Research output: Contribution to journalArticle

Abstract

The quality of the microcirculation of the uterus is a key factor for the condition of the fetus. Presently, no model is available to study directly the in vivo microciculation of the myometrium under physiological and pathophysiological circumstances. Therefore, we present a novel model to study the microcirculation and intracellular processes in the myometrium of the close to term pregnant anesthetized rats. Pups are removed from the uterus, and a small strip of uterus is prepared free, flipped over and mounted in a superfusion chamber, leaving the radix and thereby the circulation intact. Patency of the preparation was verified with RBC velocity measurements. For fluorescence ratio measurements, Indo-1 AM was used. Fluorescence images were recorded using a cooled digital CCD camera. Pixel-to-pixel ratios of the images collected at 400 nm and 506 nm were calculated to study intracellular free calcium changes. In four separate interventions, the uterus was challenged with 5×10-4 IU/ml oxytocin, 4.5 mM calcium, 5×10-4 IU/ml oxytocin with 4.5 mM calcium, and 5 ìM Ionomycin, which resulted in an increase of 400/506 nm ratio by 27%, 31%, 76% andi 103%, respectively. The model allows for semi-simultaneous measurements of red blood cell velocity and intracellular ionic and metabolic processes.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998

Fingerprint

Microcirculation
Myometrium
uterus
Uterus
Muscle
Oxytocin
Calcium
myometrium
calcium
muscles
oxytocin
Pixels
Fluorescence
Ionomycin
erythrocytes
Digital cameras
fluorescence
CCD cameras
Velocity measurement
Rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ruttner, Z., Ivanics, T., Kozma, F., Slaaf, D. W., Reneman, R. S., Ligeti, L., & Tóth, A. (1998). Microcirculation and intracellular free calcium changes in the pregnant uterine muscle. FASEB Journal, 12(5).

Microcirculation and intracellular free calcium changes in the pregnant uterine muscle. / Ruttner, Z.; Ivanics, T.; Kozma, F.; Slaaf, D. W.; Reneman, R. S.; Ligeti, L.; Tóth, A.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Ruttner, Z, Ivanics, T, Kozma, F, Slaaf, DW, Reneman, RS, Ligeti, L & Tóth, A 1998, 'Microcirculation and intracellular free calcium changes in the pregnant uterine muscle', FASEB Journal, vol. 12, no. 5.
Ruttner Z, Ivanics T, Kozma F, Slaaf DW, Reneman RS, Ligeti L et al. Microcirculation and intracellular free calcium changes in the pregnant uterine muscle. FASEB Journal. 1998 Mar 20;12(5).
Ruttner, Z. ; Ivanics, T. ; Kozma, F. ; Slaaf, D. W. ; Reneman, R. S. ; Ligeti, L. ; Tóth, A. / Microcirculation and intracellular free calcium changes in the pregnant uterine muscle. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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