This chapter discusses the microchemistry of microdissected hypothalamic nuclear areas. The hypothalamus has been considered to be the source of neuronal peptides, hormones, and neurotransmitters that influence the pituitary gland and other target tissues outside the brain. Several important technical advances have enabled the visualization of the cells producing the hormones and transmitter amines. It has also become possible to measure concentrations of neuronal peptides and neurotransmitters, as well as the activity of several enzymes, in small pieces of tissue no larger than an individual hypothalamic cell group. This development of biochemical and microdissecting techniques calls for further topographical knowledge of the hypothalamus. This chapter presents a detailed description of the hypothalamic areas and the topographical distribution of various substances measured in microdissected hypothalamic nuclei. The hypothalamus can be divided into three parts: anterior, middle, and posterior. Although the preoptic region and the mammillary body actually are not part of the hypothalamus, they have been included in several descriptions of the organ.
ASJC Scopus subject areas
- Cell Biology