Microcalorimetric study on effect of chromium(III) and chromium(VI) species on the growth of Escherichia coli

Jun Yao, Lin Tian, Fei Wang, Hui Lun Chen, Chao Qian Xu, Chun Li Su, Ming Fa Cai, Thomas Maskow, Gyula Zaray, Yan Xin Wang

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Abstract

The effects of Cr(III) and Cr(VI) species (Cr2O7 2- , CrO42- and Cr3+) on the growth of Escherichia coli (E. coli) have been investigated in detail by microcalorimetry at 37°C. Parameters including the growth rate constant (k), inhibitory ratio (I), half-inhibitory concentration (IC50), total heat output (Qtotal), time of the maximum heat production (t log) in the log phase have been obtained. The results showed that Cr(VI) and Cr(III) had the inhibition effect on the growth of E. coli in aquatic environment; however, the inhibitory ratio of Cr(III) to E. coli was smaller than that of Cr(VI). The k values of E. coli in the presence of Cr(VI) and at high concentrations of Cr(III) were decreased with increasing the concentrations of these chromium species. Among the three chromium species investigated, Cr2O72- was found to be the most poisonous species against E. coli with an IC50 value of 35.52 μg·mL-1. CrO42- exhibited moderate toxicity on E. coli with an IC50 of 50.24 μg·mL -1, and Cr3+ had the lowest toxicity with an IC 50 of 84.30 μg·mL-1. Microcalorimetry can provide a convenient, sensitive and reliable method to study the effect of various metal species on the growth of bacteria or other microorganisms.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalChinese Journal of Chemistry
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Chromium species
  • Escherichia coli
  • Metabolic action
  • Microcalorimetry
  • Toxicity

ASJC Scopus subject areas

  • Chemistry(all)

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    Yao, J., Tian, L., Wang, F., Chen, H. L., Xu, C. Q., Su, C. L., Cai, M. F., Maskow, T., Zaray, G., & Wang, Y. X. (2008). Microcalorimetric study on effect of chromium(III) and chromium(VI) species on the growth of Escherichia coli. Chinese Journal of Chemistry, 26(1), 101-106. https://doi.org/10.1002/cjoc.200890000