Microcalorimetric investigation of the toxic action of ammonium ferric(III) sulfate on the metabolic activity of pure microbes

Fei Wang, Jun Yao, Lin Tian, Yong Zhou, Huilun Chen, Haiyan Chen, Nan Gai, Yanjiao Chen, Rensheng Zhuang, G. Záray, Thomas Maskow, Emilia Bramanti

Research output: Contribution to journalArticle

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Abstract

A series of calorimetric experiments were performed to investigate toxic action of ammonium ferric sulfate (AFS) on Bacillus subtilis, Pseudomonas putida and Candida humicola. The power-time curves of micro-organism metabolism were obtained, and the action of them by addition of AFS was studied. C. humicola, B. subtilis and P. putida were inhibited completely when the concentrations were up to 320.0, 160.0 and 160.0 μg mL-1, respectively. The relationships between growth rate constant (k) and doses of AFS were approximately linear for three microbes, P. putida for 10.0-160.0 μg mL-1 (R = -0.9746), B. subtilis for 0-160.0 μg mL-1 (R = -0.9868) and C. humicola for 10.0-320.0 μg mL-1 (R = -0.9955). The total heat dissipated per milliliter (QT) for three microbes remained balance approximately during the lower doses, P. putida and B. subtilis less than the dose of 20.0 μg mL-1, 0.56 ± 0.01 and 0.26 ± 0.01 J mL-1, respectively, C. humicola less than the dose of 40.0 μg mL-1, 0.58 ± 0.03 J mL-1. The biomass and OD600 of three micro-organisms growth in the absence of AFS also were obtained. The power-time curve of C. humicola growth coincided with its turbidity curve. It elucidates that microcalorimetric method agreed with the routine microbiology method.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalEnvironmental Toxicology and Pharmacology
Volume25
Issue number3
DOIs
Publication statusPublished - May 2008

Fingerprint

Toxic Actions
Pseudomonas putida
Bacillus subtilis
Ammonium Compounds
Sulfates
Growth
Microbiology
Candida
Bacilli
Turbidity
Metabolism
Biomass
Enthalpy
Rate constants
Hot Temperature
ammonium ferric sulfate
Experiments

Keywords

  • Ammonium ferric(III) sulfate
  • Bacillus subtilis
  • Candida humicola
  • Microcalorimetry
  • Pseudomonas putida
  • Toxic action

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Microcalorimetric investigation of the toxic action of ammonium ferric(III) sulfate on the metabolic activity of pure microbes. / Wang, Fei; Yao, Jun; Tian, Lin; Zhou, Yong; Chen, Huilun; Chen, Haiyan; Gai, Nan; Chen, Yanjiao; Zhuang, Rensheng; Záray, G.; Maskow, Thomas; Bramanti, Emilia.

In: Environmental Toxicology and Pharmacology, Vol. 25, No. 3, 05.2008, p. 351-357.

Research output: Contribution to journalArticle

Wang, Fei ; Yao, Jun ; Tian, Lin ; Zhou, Yong ; Chen, Huilun ; Chen, Haiyan ; Gai, Nan ; Chen, Yanjiao ; Zhuang, Rensheng ; Záray, G. ; Maskow, Thomas ; Bramanti, Emilia. / Microcalorimetric investigation of the toxic action of ammonium ferric(III) sulfate on the metabolic activity of pure microbes. In: Environmental Toxicology and Pharmacology. 2008 ; Vol. 25, No. 3. pp. 351-357.
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AU - Chen, Haiyan

AU - Gai, Nan

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AU - Maskow, Thomas

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