Microbial synthesis of chitinase in solid cultures and its potential as a biocontrol agent against phytopathogenic fungus Colletotrichum gloeosporioides

Chandran Sandhya, Parameswaran Binod, K. Madhavan Nampoothiri, George Szakacs, Ashok Pandey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Antifungal activity of chitinase can be effectively utilized in biologic pest control strategies. Because solid-state cultivation has been termed a cost-effective means for fungal growth and metabolite production, chitinase production by Trichoderma harzianum was studied using wheat bran-based solid medium containing 1 % colloidal chitin. Chitinase synthesis was found to be growth associated because maximum enzyme (5.4 U/g of dry substrate) and biomass production occurred at 72 h. Substrate moisture had a critical impact on chitinase production; five grams of medium having an initial moisture content of 68.4% when incubated for 72 h increased the enzyme yield to 9.3 U/g of dry substrate. Optimization of colloidal chitin concentration showed that improvements in chitinase yield and maximum activity were attained with a 2% (w/w) concentration. Supplementation of additional nitrogen sources also influenced enzyme production, and the best yield was obtained with yeast extract. The effect of crude chitinase on hyphal morphology of the phytopathogenic fungus Colletotrichum gloeosporioides was swelling as well as lysis of hyphal wall, depending on the age of the mycelium. Studies of pH and thermal stability showed that crude culture filtrate was active over pH 4.0-6.0 and retained about 48.2% activity after 40 min of incubation at 40°C.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume127
Issue number1
Publication statusPublished - Oct 2005

Fingerprint

Biocontrol
Colletotrichum
Chitinases
Fungi
Chitin
Enzymes
Substrates
Moisture
Pest control
Biological Pest Control
Metabolites
Trichoderma
Yeast
Mycelium
Dietary Fiber
Swelling
Growth
Biomass
Thermodynamic stability
Nitrogen

Keywords

  • Antifungal activity
  • Biologic control
  • Chitinase
  • pH stability
  • Solid-state cultivation
  • Thermal stability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Microbial synthesis of chitinase in solid cultures and its potential as a biocontrol agent against phytopathogenic fungus Colletotrichum gloeosporioides. / Sandhya, Chandran; Binod, Parameswaran; Nampoothiri, K. Madhavan; Szakacs, George; Pandey, Ashok.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 127, No. 1, 10.2005, p. 1-15.

Research output: Contribution to journalArticle

@article{85286ce1e14e42e0a9536a7c6417ffa0,
title = "Microbial synthesis of chitinase in solid cultures and its potential as a biocontrol agent against phytopathogenic fungus Colletotrichum gloeosporioides",
abstract = "Antifungal activity of chitinase can be effectively utilized in biologic pest control strategies. Because solid-state cultivation has been termed a cost-effective means for fungal growth and metabolite production, chitinase production by Trichoderma harzianum was studied using wheat bran-based solid medium containing 1 {\%} colloidal chitin. Chitinase synthesis was found to be growth associated because maximum enzyme (5.4 U/g of dry substrate) and biomass production occurred at 72 h. Substrate moisture had a critical impact on chitinase production; five grams of medium having an initial moisture content of 68.4{\%} when incubated for 72 h increased the enzyme yield to 9.3 U/g of dry substrate. Optimization of colloidal chitin concentration showed that improvements in chitinase yield and maximum activity were attained with a 2{\%} (w/w) concentration. Supplementation of additional nitrogen sources also influenced enzyme production, and the best yield was obtained with yeast extract. The effect of crude chitinase on hyphal morphology of the phytopathogenic fungus Colletotrichum gloeosporioides was swelling as well as lysis of hyphal wall, depending on the age of the mycelium. Studies of pH and thermal stability showed that crude culture filtrate was active over pH 4.0-6.0 and retained about 48.2{\%} activity after 40 min of incubation at 40°C.",
keywords = "Antifungal activity, Biologic control, Chitinase, pH stability, Solid-state cultivation, Thermal stability",
author = "Chandran Sandhya and Parameswaran Binod and Nampoothiri, {K. Madhavan} and George Szakacs and Ashok Pandey",
year = "2005",
month = "10",
language = "English",
volume = "127",
pages = "1--15",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Humana Press",
number = "1",

}

TY - JOUR

T1 - Microbial synthesis of chitinase in solid cultures and its potential as a biocontrol agent against phytopathogenic fungus Colletotrichum gloeosporioides

AU - Sandhya, Chandran

AU - Binod, Parameswaran

AU - Nampoothiri, K. Madhavan

AU - Szakacs, George

AU - Pandey, Ashok

PY - 2005/10

Y1 - 2005/10

N2 - Antifungal activity of chitinase can be effectively utilized in biologic pest control strategies. Because solid-state cultivation has been termed a cost-effective means for fungal growth and metabolite production, chitinase production by Trichoderma harzianum was studied using wheat bran-based solid medium containing 1 % colloidal chitin. Chitinase synthesis was found to be growth associated because maximum enzyme (5.4 U/g of dry substrate) and biomass production occurred at 72 h. Substrate moisture had a critical impact on chitinase production; five grams of medium having an initial moisture content of 68.4% when incubated for 72 h increased the enzyme yield to 9.3 U/g of dry substrate. Optimization of colloidal chitin concentration showed that improvements in chitinase yield and maximum activity were attained with a 2% (w/w) concentration. Supplementation of additional nitrogen sources also influenced enzyme production, and the best yield was obtained with yeast extract. The effect of crude chitinase on hyphal morphology of the phytopathogenic fungus Colletotrichum gloeosporioides was swelling as well as lysis of hyphal wall, depending on the age of the mycelium. Studies of pH and thermal stability showed that crude culture filtrate was active over pH 4.0-6.0 and retained about 48.2% activity after 40 min of incubation at 40°C.

AB - Antifungal activity of chitinase can be effectively utilized in biologic pest control strategies. Because solid-state cultivation has been termed a cost-effective means for fungal growth and metabolite production, chitinase production by Trichoderma harzianum was studied using wheat bran-based solid medium containing 1 % colloidal chitin. Chitinase synthesis was found to be growth associated because maximum enzyme (5.4 U/g of dry substrate) and biomass production occurred at 72 h. Substrate moisture had a critical impact on chitinase production; five grams of medium having an initial moisture content of 68.4% when incubated for 72 h increased the enzyme yield to 9.3 U/g of dry substrate. Optimization of colloidal chitin concentration showed that improvements in chitinase yield and maximum activity were attained with a 2% (w/w) concentration. Supplementation of additional nitrogen sources also influenced enzyme production, and the best yield was obtained with yeast extract. The effect of crude chitinase on hyphal morphology of the phytopathogenic fungus Colletotrichum gloeosporioides was swelling as well as lysis of hyphal wall, depending on the age of the mycelium. Studies of pH and thermal stability showed that crude culture filtrate was active over pH 4.0-6.0 and retained about 48.2% activity after 40 min of incubation at 40°C.

KW - Antifungal activity

KW - Biologic control

KW - Chitinase

KW - pH stability

KW - Solid-state cultivation

KW - Thermal stability

UR - http://www.scopus.com/inward/record.url?scp=25444465681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444465681&partnerID=8YFLogxK

M3 - Article

C2 - 16186619

AN - SCOPUS:25444465681

VL - 127

SP - 1

EP - 15

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1

ER -