Microanalytical investigation of nematodes

Zoltán Sávoly, Péter Nagy, K. Havancsák, G. Záray

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The chemical characterization of individual free-living nematodes is a challenge due to their small size and correspondingly, their low dry mass (1-3μg). The concentrations of minor (S, P, Ca, K) and trace elements (Fe, Zn, Cu, Ni, and Mn) of individual specimens (Xiphinema Vuittenezi) were determined by total reflection X-ray fluorescence (TXRF) spectrometry following a two step sample preparation procedure. The mean concentrations of minor and trace elements changed in the range of 1-4 and 0.01-0.4ng/μg, respectively. The relative standard deviation varied between 9.4 and 25.3% which was influenced not only by the analytical procedure but also by the biological deviations among the eleven investigated specimens. The stress effect caused by CuSO 4 treatment resulted in significant change in the nematodes only in their Zn content. In the lack of certified reference material, the TXRF method was checked by inductively coupled plasma mass spectrometry (ICP-MS) analyzing solutions of 100 digested nematodes. The carbon and nitrogen content of nematodes was also measured by total C/N analyzer and their ratio amounted to 4.78±0.31 which is lower than the values published for the bacterial or fungal feeding ones. The way of copper uptake was investigated by scanning electron microscopy (SEM). Nematode treated with copper-nitrate was pecked out at the mid-body using focused ion beam (FIB) technique. In order to preserve the cross-section to be investigated, platinum coating was applied prior to the FIB operation. Energy dispersive X-ray analysis was performed along the cross-section measuring the distribution of platinum, sulfur and copper. It has been found that the sulfur and copper distributions were fairly similar following the copper treatment, and these elements had the highest concentration in the cuticle.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalMicrochemical Journal
Volume105
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Copper
Focused ion beams
Trace Elements
Platinum
Sulfur
Inductively coupled plasma mass spectrometry
Energy dispersive X ray analysis
Nitrates
Nitrogen
Carbon
Fluorescence
X rays
Coatings
Scanning electron microscopy

Keywords

  • Elemental distribution
  • Focused ion beam-scanning electron microscopy
  • Inductively coupled plasma mass spectrometry
  • Nematodes
  • Total reflection X-ray fluorescence spectrometry
  • Xiphinema vuittenezi

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Microanalytical investigation of nematodes. / Sávoly, Zoltán; Nagy, Péter; Havancsák, K.; Záray, G.

In: Microchemical Journal, Vol. 105, 11.2012, p. 83-87.

Research output: Contribution to journalArticle

Sávoly, Zoltán ; Nagy, Péter ; Havancsák, K. ; Záray, G. / Microanalytical investigation of nematodes. In: Microchemical Journal. 2012 ; Vol. 105. pp. 83-87.
@article{66e35e0247b4458182c70b58788d820f,
title = "Microanalytical investigation of nematodes",
abstract = "The chemical characterization of individual free-living nematodes is a challenge due to their small size and correspondingly, their low dry mass (1-3μg). The concentrations of minor (S, P, Ca, K) and trace elements (Fe, Zn, Cu, Ni, and Mn) of individual specimens (Xiphinema Vuittenezi) were determined by total reflection X-ray fluorescence (TXRF) spectrometry following a two step sample preparation procedure. The mean concentrations of minor and trace elements changed in the range of 1-4 and 0.01-0.4ng/μg, respectively. The relative standard deviation varied between 9.4 and 25.3{\%} which was influenced not only by the analytical procedure but also by the biological deviations among the eleven investigated specimens. The stress effect caused by CuSO 4 treatment resulted in significant change in the nematodes only in their Zn content. In the lack of certified reference material, the TXRF method was checked by inductively coupled plasma mass spectrometry (ICP-MS) analyzing solutions of 100 digested nematodes. The carbon and nitrogen content of nematodes was also measured by total C/N analyzer and their ratio amounted to 4.78±0.31 which is lower than the values published for the bacterial or fungal feeding ones. The way of copper uptake was investigated by scanning electron microscopy (SEM). Nematode treated with copper-nitrate was pecked out at the mid-body using focused ion beam (FIB) technique. In order to preserve the cross-section to be investigated, platinum coating was applied prior to the FIB operation. Energy dispersive X-ray analysis was performed along the cross-section measuring the distribution of platinum, sulfur and copper. It has been found that the sulfur and copper distributions were fairly similar following the copper treatment, and these elements had the highest concentration in the cuticle.",
keywords = "Elemental distribution, Focused ion beam-scanning electron microscopy, Inductively coupled plasma mass spectrometry, Nematodes, Total reflection X-ray fluorescence spectrometry, Xiphinema vuittenezi",
author = "Zolt{\'a}n S{\'a}voly and P{\'e}ter Nagy and K. Havancs{\'a}k and G. Z{\'a}ray",
year = "2012",
month = "11",
doi = "10.1016/j.microc.2012.03.021",
language = "English",
volume = "105",
pages = "83--87",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Microanalytical investigation of nematodes

AU - Sávoly, Zoltán

AU - Nagy, Péter

AU - Havancsák, K.

AU - Záray, G.

PY - 2012/11

Y1 - 2012/11

N2 - The chemical characterization of individual free-living nematodes is a challenge due to their small size and correspondingly, their low dry mass (1-3μg). The concentrations of minor (S, P, Ca, K) and trace elements (Fe, Zn, Cu, Ni, and Mn) of individual specimens (Xiphinema Vuittenezi) were determined by total reflection X-ray fluorescence (TXRF) spectrometry following a two step sample preparation procedure. The mean concentrations of minor and trace elements changed in the range of 1-4 and 0.01-0.4ng/μg, respectively. The relative standard deviation varied between 9.4 and 25.3% which was influenced not only by the analytical procedure but also by the biological deviations among the eleven investigated specimens. The stress effect caused by CuSO 4 treatment resulted in significant change in the nematodes only in their Zn content. In the lack of certified reference material, the TXRF method was checked by inductively coupled plasma mass spectrometry (ICP-MS) analyzing solutions of 100 digested nematodes. The carbon and nitrogen content of nematodes was also measured by total C/N analyzer and their ratio amounted to 4.78±0.31 which is lower than the values published for the bacterial or fungal feeding ones. The way of copper uptake was investigated by scanning electron microscopy (SEM). Nematode treated with copper-nitrate was pecked out at the mid-body using focused ion beam (FIB) technique. In order to preserve the cross-section to be investigated, platinum coating was applied prior to the FIB operation. Energy dispersive X-ray analysis was performed along the cross-section measuring the distribution of platinum, sulfur and copper. It has been found that the sulfur and copper distributions were fairly similar following the copper treatment, and these elements had the highest concentration in the cuticle.

AB - The chemical characterization of individual free-living nematodes is a challenge due to their small size and correspondingly, their low dry mass (1-3μg). The concentrations of minor (S, P, Ca, K) and trace elements (Fe, Zn, Cu, Ni, and Mn) of individual specimens (Xiphinema Vuittenezi) were determined by total reflection X-ray fluorescence (TXRF) spectrometry following a two step sample preparation procedure. The mean concentrations of minor and trace elements changed in the range of 1-4 and 0.01-0.4ng/μg, respectively. The relative standard deviation varied between 9.4 and 25.3% which was influenced not only by the analytical procedure but also by the biological deviations among the eleven investigated specimens. The stress effect caused by CuSO 4 treatment resulted in significant change in the nematodes only in their Zn content. In the lack of certified reference material, the TXRF method was checked by inductively coupled plasma mass spectrometry (ICP-MS) analyzing solutions of 100 digested nematodes. The carbon and nitrogen content of nematodes was also measured by total C/N analyzer and their ratio amounted to 4.78±0.31 which is lower than the values published for the bacterial or fungal feeding ones. The way of copper uptake was investigated by scanning electron microscopy (SEM). Nematode treated with copper-nitrate was pecked out at the mid-body using focused ion beam (FIB) technique. In order to preserve the cross-section to be investigated, platinum coating was applied prior to the FIB operation. Energy dispersive X-ray analysis was performed along the cross-section measuring the distribution of platinum, sulfur and copper. It has been found that the sulfur and copper distributions were fairly similar following the copper treatment, and these elements had the highest concentration in the cuticle.

KW - Elemental distribution

KW - Focused ion beam-scanning electron microscopy

KW - Inductively coupled plasma mass spectrometry

KW - Nematodes

KW - Total reflection X-ray fluorescence spectrometry

KW - Xiphinema vuittenezi

UR - http://www.scopus.com/inward/record.url?scp=84867887892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867887892&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2012.03.021

DO - 10.1016/j.microc.2012.03.021

M3 - Article

VL - 105

SP - 83

EP - 87

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

ER -