Microalbuminuria, indicated by total versus immunoreactive urinary albumins, in acute ischemic stroke patients

Peter Toth, Akos Koller, Gabriella Pusch, Edit Bosnyak, Laszlo Szapary, Samuel Komoly, Lajos Marko, Judit Nagy, Istvan Wittmann

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Microalbuminuria, assessed by measuring immunoreactive albumin, is common in patients with cerebrovascular disease and is associated with increased risk of stroke. Total urinary albumin (t-uAlb) comprises both immunoreactive albumin (ir-uAlb) and nonimmunoreactive albumin (nir-uAlb). We hypothesized that t-uAlb is a more sensitive indicator of microalbuminuria than ir-uAlb, and that measurement of t-uAlb will increase the prevalence of microalbuminuria in ischemic stroke patients compared with measurement of ir-uAlb and will show a stronger correlation with the severity of stroke and oxidative stress. In urine samples from 98 patients with ischemic stroke, the albumin-to-creatinine ratios t-uAlb/uCreat and ir-uAlb/uCreat were measured by high-performance liquid chromatography (HPLC) and immunoturbidimetry (IT), and the nir-uAlb/uCreat ratio was calculated. Urinary ortho-tyrosine (o-Tyr/uCreat), an indicator of oxidative stress, was measured by HPLC. The severity of stroke was scored based on the National Institutes of Health Stroke Scale (NIHSS). The prevalence of microalbuminuria detected by HPLC was significantly higher than that detetcted by IT (66.3 vs 36.7%). Although all forms of albumin showed significant correlation with stroke severity (t-uAlb: r = 0.24, P <.05 ir-uAlb: r = 0.25, P <.05 nir-uAlb: r = 0.29, P <.05), only nir-uAlb was found to be an independent predictor of stroke severity (B = 0.20, β = 0.35, P <.05). In addition, t-uAlb/uCreat and nir-uAlb/uCreat had a significant correlation with o-Tyr/uCreat (r = 0.336, P <.05 and r = 0.358, P <.05 respectively), whereas ir-uAlb/uCreat did not (r = 0.22, P >.05). Our data suggest that in acute ischemic stroke patients, t-uAlb is a more sensitive indicator of microalbuminuria than the presently used ir-uAlb. Future studies should aim to elucidate the underlying mechanisms for the relationship among urinary albumins and cerebrovascular diseases and the role of urinary albumins in risk stratification for stroke.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalJournal of Stroke and Cerebrovascular Diseases
Volume20
Issue number6
DOIs
Publication statusPublished - Nov 1 2011

Keywords

  • Microvascular diseases
  • National Institutes of Health Stroke Severity Scale
  • endothelial dysfunction
  • oxidative stress
  • prevention of stroke
  • stroke

ASJC Scopus subject areas

  • Surgery
  • Rehabilitation
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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