Micro-preparative OPLC - Rapid isolation by transfusion and infusion-transfusion processes

E. Mincsovics, Éva Sárdi, István Velich, G. Kátay, E. Tyihák

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A new OPLC procedure, infusion-transfusion OPLC, has been developed and compared with conventional transfusion OPLC. Spot and/or band deformation caused by the total wetness front (which results from pore filling) was reduced, as was the bubble effect in on-line detection. Both techniques were used for rapid micro-preparative OPLC isolation on analytical adsorbent layers. In-situ clean-up and separation were used to isolate trigonelline from Leuzea extract. Modeling of loading capacity for isolation of ascorbigen was accomplished by fully off-line OPLC. Under optimized conditions ascorbigen of high purity was isolated from cabbage extract by transfusion and infusion-transfusion OPLC.

Original languageEnglish
Pages (from-to)280-285
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume15
Issue number4
Publication statusPublished - Jul 2002

Fingerprint

Leuzea
Brassica
Adsorbents
ascorbigen
trigonelline

Keywords

  • Ascorbigen
  • Infusion OPLC
  • Infusion-tranfusion OPLC
  • Optimum-performance laminar chromatography, OPLC
  • Over-pressured layer chromatography, OPLC
  • Transfusion OPLC
  • Trigonelline

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Micro-preparative OPLC - Rapid isolation by transfusion and infusion-transfusion processes. / Mincsovics, E.; Sárdi, Éva; Velich, István; Kátay, G.; Tyihák, E.

In: Journal of Planar Chromatography - Modern TLC, Vol. 15, No. 4, 07.2002, p. 280-285.

Research output: Contribution to journalArticle

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