Micro-ERDA, micro-RBS and micro-PIXE techniques in the investigation of fish otoliths

R. Huszank, A. Simon, E. Szilágyi, K. Keresztessy, I. Kovács

Research output: Contribution to journalArticle

15 Citations (Scopus)


Elemental distribution in the otolith of the fresh water fish burbot (Lota lota L.) collected in Hungary was measured with Elastic Recoil Detection Analysis (ERDA), Rutherford Backscattering Spectrometry (RBS) and as a complementary technique, Particle-Induced X-ray Emission (PIXE) with a focussed ion beam of 1.5 × 1.5 μm2 spot size. The organic- and inorganic-rich regions of the otolith are distinguished and they are presented as hydrogen and calcium maps at depth regions of 0-70, 70-140 and 140-210 nm. The textured surface of the sample and its porosity were characterized from the effect on the RBS spectra. The oxygen and carbon PIXE elemental maps can also be used to identify the organic- and inorganic-rich regions of the otolith. The calcium map was found to be more homogeneous because the otolith structure is averaged in a larger depth. The trace elements Fe, Zn and Sr were detected only in very low concentration by micro-PIXE.

Original languageEnglish
Pages (from-to)2132-2135
Number of pages4
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Issue number12-13
Publication statusPublished - Jun 15 2009


  • ERDA
  • Elemental depth profiling
  • Otolith
  • PIXE
  • RBS
  • Scanning nuclear microprobe

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

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