Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytes

J. J. Hedberg, R. C. Grafström, M. Vondracek, Z. Sarang, L. Wärngård, J. O. Höög

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Enzymes involved in various protective and metabolic processes of carbonyl compounds were analysed utilising a micro-array method in a three-stage in vitro model for oral carcinogenesis involving cultured normal, immortalised and malignant human oral keratinocytes. A complete transcript profiling of identified carbonyl-metabolising enzymes belonging to the ADH, ALDH, SDR and AKR families is presented. Expression of 17 transcripts was detected in normal, 14 in immortalized and 19 in malignant keratinocytes of a total of 12,500 genes spotted on the micro-array chip. For the detected transcripts, about half were changed by cell transformation, and for the various enzyme families, differences in expression patterns were observed. The detected AKR transcripts displayed a conserved pattern of expression, indicating a requirement for the keratinocyte phenotype, while most of the detected SDRs displayed changed expression at the various stages of malignancy. The importance of multiple experiments in using a microarray technique for reliable results is underlined and, finally, the strength of the method in detecting co-expressed enzymes in metabolic pathways is exemplified by the detection of the formaldehyde-scavenging pathway enzymes and the polyol pathway enzymes.

Original languageEnglish
Pages (from-to)1719-1726
Number of pages8
JournalCellular and Molecular Life Sciences
Volume58
Issue number11
Publication statusPublished - 2001

Fingerprint

Keratinocytes
Enzymes
Carbonyl compounds
Scavenging
Microarrays
Metabolic Networks and Pathways
Formaldehyde
Carcinogenesis
Genes
Phenotype
Neoplasms
Experiments

Keywords

  • Alcohol dehydrogenase
  • Aldehyde dehydrogenase
  • Carbonyl-metabolising enzymes
  • Micro-array chip

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Hedberg, J. J., Grafström, R. C., Vondracek, M., Sarang, Z., Wärngård, L., & Höög, J. O. (2001). Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytes. Cellular and Molecular Life Sciences, 58(11), 1719-1726.

Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytes. / Hedberg, J. J.; Grafström, R. C.; Vondracek, M.; Sarang, Z.; Wärngård, L.; Höög, J. O.

In: Cellular and Molecular Life Sciences, Vol. 58, No. 11, 2001, p. 1719-1726.

Research output: Contribution to journalArticle

Hedberg, JJ, Grafström, RC, Vondracek, M, Sarang, Z, Wärngård, L & Höög, JO 2001, 'Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytes', Cellular and Molecular Life Sciences, vol. 58, no. 11, pp. 1719-1726.
Hedberg, J. J. ; Grafström, R. C. ; Vondracek, M. ; Sarang, Z. ; Wärngård, L. ; Höög, J. O. / Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytes. In: Cellular and Molecular Life Sciences. 2001 ; Vol. 58, No. 11. pp. 1719-1726.
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