Micellar electrokinetic chromatography of highly hydrophobic peptides

M. Idei, J. Seprodi, E. Gyorffy, F. Hollósy, Zs Vadász, Gy Mészáros, I. Teplán, Gy Kéri

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A micellar electrokinetic chromatography (MEKC) separation method was developed for analysis of highly hydrophobic derivatized peptides that are able to reverse multidrug resistance (MDR) observed in chemotherapy of human tumors. The hydrophobicity of these peptides was characterized by the retention factor (k(MEKC)') obtained by MEKC and by the k(HPLC)' obtained by reversed phase high performance liquid chromatography (RP-HPLC). A tendency was observed between the logk(MEKC)' values of the peptides investigated and their capabilities to reverse MDR. The higher the k(MEKC) value of the peptide, the higher its hydrophobicity and the higher was its capability to reverse MDR. This result was in accordance with that obtained by RP-HPLC. Therefore, the effect of the peptides on MDR could be estimated on the basis of their logk(MEKC)' values. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalAnalytica Chimica Acta
Volume372
Issue number1-2
DOIs
Publication statusPublished - Oct 19 1998

    Fingerprint

Keywords

  • Capillary electrophoresis
  • Hydrophobicity
  • Micellar electrokinetic chromatography
  • Multidrug resistance
  • Peptide synthesis
  • Retention-activity relationship

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this