Mice lacking the extracellular matrix adaptor protein matrilin-2 develop without obvious abnormalities

L. Mátés, Claudia Nicolae, Matthias Mörgelin, Ferenc Deák, I. Kiss, Attila Aszódi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Matrilins are putative adaptor proteins of the extracellular matrix (ECM) which can form both collagen-dependent and collagen-independent filamentous networks. While all known matrilins (matrilin-1, -2, -3, and -4) are expressed in cartilage, only matrilin-2 and matrilin-4 are abundant in non-skeletal tissues. To clarify the biological role of matrilin-2, we have developed a matrilin-2-deficient mouse strain. Matrilin-2 null mice show no gross abnormalities during embryonic or adult development, are fertile, and have a normal lifespan. Histological and ultrastructural analyses indicate apparently normal structure of all organs and tissues where matrilin-2 is expressed. Although matrilin-2 co-localizes with matrilin-4 in many tissues, Northern hybridization, semiquantitative RT-PCR, immunohistochemistry and biochemical analysis reveal no significant alteration in the steady-state level of matrilin-4 expression in homozygous mutant mice. Immunostaining of wild-type and mutant skin samples indicate no detectable differences in the expression and deposition of matrilin-2 binding partners including collagen I, laminin-nidogen complexes, fibrillin-2 and fibronectin. In addition, electron microscopy reveals an intact basement membrane at the epidermal-dermal junction and normal organization of the dermal collagen fibrils in mutant skin. These data suggest that either matrilin-2 and matrilin-2-mediated matrix-matrix interactions are dispensable for proper ECM assembly and function, or that they are efficiently compensated by other matrix components including wild-type levels of matrilin-4.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalMatrix Biology
Volume23
Issue number3
DOIs
Publication statusPublished - Jun 2004

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Matrilin Proteins
Extracellular Matrix Proteins
Collagen
Skin

Keywords

  • Cartilage
  • Extracellular matrix
  • Matrilin
  • Skin

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Mice lacking the extracellular matrix adaptor protein matrilin-2 develop without obvious abnormalities. / Mátés, L.; Nicolae, Claudia; Mörgelin, Matthias; Deák, Ferenc; Kiss, I.; Aszódi, Attila.

In: Matrix Biology, Vol. 23, No. 3, 06.2004, p. 195-204.

Research output: Contribution to journalArticle

Mátés, L. ; Nicolae, Claudia ; Mörgelin, Matthias ; Deák, Ferenc ; Kiss, I. ; Aszódi, Attila. / Mice lacking the extracellular matrix adaptor protein matrilin-2 develop without obvious abnormalities. In: Matrix Biology. 2004 ; Vol. 23, No. 3. pp. 195-204.
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