Mice deficient in pituitary adenylate cyclase activating polypeptide (PACAP) are more susceptible to retinal ischemic injury in vivo

K. Szabadfi, T. Atlasz, P. Kiss, B. Danyadi, A. Tamás, Z. Helyes, H. Hashimoto, N. Shintani, A. Baba, G. Tóth, R. Gábriel, D. Reglodi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuroprotective peptide exerting protective effects in neuronal injuries. We have provided evidence that PACAP is neuroprotective in several models of retinal degeneration in vivo. Our previous studies showed that PACAP treatment ameliorated the damaging effects of chronic hypoperfusion modeled by permanent bilateral carotid artery occlusion. We have also demonstrated in earlier studies that treatment with PACAP antagonists further aggravates retinal lesions. It has been shown that PACAP deficient mice have larger infarct size in cerebral ischemia. The aim of this study was to compare the degree of retinal damage in wild type and PACAP deficient mice in ischemic retinal insult. Mice underwent 10 min of bilateral carotid artery occlusion followed by 2-week reperfusion period. Retinas were then processed for histological analysis. It was found that PACAP deficient mice had significantly greater retinal damage, as shown by the thickness of the whole retina, the morphometric analysis of the individual retinal layers, and the cell numbers in the inner nuclear and ganglion cell layers. Exogenous PACAP administration could partially protect against retinal degeneration in PACAP deficient mice. These results clearly show that endogenous PACAP reacts as a stress-response peptide that is necessary for endogenous protection against different retinal insults.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalNeurotoxicity Research
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 2012

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Pituitary Adenylate Cyclase-Activating Polypeptide
Wounds and Injuries
Retinal Degeneration
Carotid Arteries
Retina
Peptides
Brain Ischemia
Ganglia
Reperfusion
Cell Count
Cells

Keywords

  • Endogenous PACAP
  • Intravitreal PACAP treatment
  • PACAP knockout
  • Retina
  • Transient ischemia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Mice deficient in pituitary adenylate cyclase activating polypeptide (PACAP) are more susceptible to retinal ischemic injury in vivo. / Szabadfi, K.; Atlasz, T.; Kiss, P.; Danyadi, B.; Tamás, A.; Helyes, Z.; Hashimoto, H.; Shintani, N.; Baba, A.; Tóth, G.; Gábriel, R.; Reglodi, D.

In: Neurotoxicity Research, Vol. 21, No. 1, 01.2012, p. 41-48.

Research output: Contribution to journalArticle

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