MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells

Gábor Mocsár, Julianna Volkó, Daniel Rönnlund, Jerker Widengren, Péter Nagy, János Szöllősi, Katalin Tóth, Carolyn K. Goldman, S. Damjanovich, Thomas A. Waldmann, Andrea Bodnár, György Vámosi

Research output: Contribution to journalArticle

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Abstract

MHC glycoproteins form supramolecular clusters with interleukin-2 and -15 receptors in lipid rafts of T cells. The role of highly expressed MHC I in maintaining these clusters is unknown. We knocked down MHC I in FT7.10 human T cells, and studied protein clustering at two hierarchic levels: molecular aggregations and mobility by Förster resonance energy transfer and fluorescence correlation spectroscopy; and segregation into larger domains or superclusters by superresolution stimulated emission depletion microscopy. Fluorescence correlation spectroscopy-based molecular brightness analysis revealed that the studied molecules diffused as tight aggregates of several proteins of a kind. Knockdown reduced the number of MHC I containing molecular aggregates and their average MHC I content, and decreased the heteroassociation of MHC I with IL-2Rα/IL-15Rα. The mobility of not only MHC I but also that of IL-2Rα/IL-15Rα increased, corroborating the general size decrease of tight aggregates. A multifaceted analysis of stimulated emission depletion images revealed that the diameter of MHC I superclusters diminished from 400–600 to 200–300 nm, whereas those of IL-2Rα/IL-15Rα hardly changed. MHC I and IL-2Rα/IL-15Rα colocalized with GM1 ganglioside-rich lipid rafts, but MHC I clusters retracted to smaller subsets of GM1- and IL-2Rα/IL-15Rα-rich areas upon knockdown. Our results prove that changes in expression level may significantly alter the organization and mobility of interacting membrane proteins.

Original languageEnglish
Pages (from-to)100-112
Number of pages13
JournalBiophysical Journal
Volume111
Issue number1
DOIs
Publication statusPublished - Jul 12 2016

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Interleukin-15 Receptors
Interleukin-2 Receptors
Fluorescence Spectrometry
Cluster Analysis
G(M1) Ganglioside
T-Lymphocytes
Lipids
Energy Transfer
Microscopy
Glycoproteins
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Mocsár, G., Volkó, J., Rönnlund, D., Widengren, J., Nagy, P., Szöllősi, J., ... Vámosi, G. (2016). MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells. Biophysical Journal, 111(1), 100-112. https://doi.org/10.1016/j.bpj.2016.05.044

MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells. / Mocsár, Gábor; Volkó, Julianna; Rönnlund, Daniel; Widengren, Jerker; Nagy, Péter; Szöllősi, János; Tóth, Katalin; Goldman, Carolyn K.; Damjanovich, S.; Waldmann, Thomas A.; Bodnár, Andrea; Vámosi, György.

In: Biophysical Journal, Vol. 111, No. 1, 12.07.2016, p. 100-112.

Research output: Contribution to journalArticle

Mocsár, G, Volkó, J, Rönnlund, D, Widengren, J, Nagy, P, Szöllősi, J, Tóth, K, Goldman, CK, Damjanovich, S, Waldmann, TA, Bodnár, A & Vámosi, G 2016, 'MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells', Biophysical Journal, vol. 111, no. 1, pp. 100-112. https://doi.org/10.1016/j.bpj.2016.05.044
Mocsár G, Volkó J, Rönnlund D, Widengren J, Nagy P, Szöllősi J et al. MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells. Biophysical Journal. 2016 Jul 12;111(1):100-112. https://doi.org/10.1016/j.bpj.2016.05.044
Mocsár, Gábor ; Volkó, Julianna ; Rönnlund, Daniel ; Widengren, Jerker ; Nagy, Péter ; Szöllősi, János ; Tóth, Katalin ; Goldman, Carolyn K. ; Damjanovich, S. ; Waldmann, Thomas A. ; Bodnár, Andrea ; Vámosi, György. / MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2 and -15 Receptors in T Cells. In: Biophysical Journal. 2016 ; Vol. 111, No. 1. pp. 100-112.
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AU - Szöllősi, János

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