Apoptózis indukálása mevastatinnal humán U266 myeloma sejtvonalon

Translated title of the contribution: Mevastatin induced apoptosis in U266 human myeloma cells

Jánosi Judit, A. Sebestyén, Bocsi József, G. Barna, Nagy Katalin, Vályi Nagy István, L. Kópper

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Statins have been used succesfully in the treatment of hypercholesterinaemia. Moreover, in vitro studies have shown that statins can trigger apoptosis in a variety of tumor cell lines. In the present study we analysed the effect of mevastatin-a novel inhibitor of HMG-COA reductase, the rate-limiting enzyme of the mevalonate pathway-on U266 human myeloma cells. Apoptosis induced by mevastatin was associated with increased caspase activity and depolarisation of mitochondrial membrane. Expression of BCL-2 mRNA and protein was downregulated, with no change in BAX or BCLxL protein production. The mitochondrial program was supported by caspase-8 and cleaved BID activity. None of the antibodies neutralising death-ligand/death-receptor pathway-TRAIL-R2Fc, anti-TNF-a, anti FASL (NOK-1)-influenced the mevastatin-induced apoptosis. Mevastatin also stimulated shedding of syndecan-1 from the surface of myeloma cells.

Original languageHungarian
Pages (from-to)333-337
Number of pages5
JournalMagyar Onkologia
Volume48
Issue number4
Publication statusPublished - 2004

Fingerprint

Apoptosis
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Syndecan-1
Mevalonic Acid
Death Domain Receptors
Caspase 8
Mitochondrial Membranes
Caspases
Neutralizing Antibodies
Tumor Cell Line
Oxidoreductases
Proteins
Down-Regulation
Ligands
Messenger RNA
mevastatin
Enzymes
In Vitro Techniques

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Apoptózis indukálása mevastatinnal humán U266 myeloma sejtvonalon. / Judit, Jánosi; Sebestyén, A.; József, Bocsi; Barna, G.; Katalin, Nagy; István, Vályi Nagy; Kópper, L.

In: Magyar Onkologia, Vol. 48, No. 4, 2004, p. 333-337.

Research output: Contribution to journalArticle

Judit, J, Sebestyén, A, József, B, Barna, G, Katalin, N, István, VN & Kópper, L 2004, 'Apoptózis indukálása mevastatinnal humán U266 myeloma sejtvonalon', Magyar Onkologia, vol. 48, no. 4, pp. 333-337.
Judit J, Sebestyén A, József B, Barna G, Katalin N, István VN et al. Apoptózis indukálása mevastatinnal humán U266 myeloma sejtvonalon. Magyar Onkologia. 2004;48(4):333-337.
Judit, Jánosi ; Sebestyén, A. ; József, Bocsi ; Barna, G. ; Katalin, Nagy ; István, Vályi Nagy ; Kópper, L. / Apoptózis indukálása mevastatinnal humán U266 myeloma sejtvonalon. In: Magyar Onkologia. 2004 ; Vol. 48, No. 4. pp. 333-337.
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