Methylated cap formation by enzymes bound to nuclear informofer particles

G. Bajszár, G. Szabó, A. Simoncsits, J. Molnár

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Rat liver nuclear 30 S ribonucleoprotein particles containing pre-mRNA and nuclear sap proteins have been shown to modify in vitro the synthetic dinucleotide ppGpC in the presence of GTP and S-adenosyl-L-methionine (SAM) by the formation of a blocked and methylated (capped) structure 7meG(5′)ppp(5′)-Gm epC. In the absence of SAM the predominant reaction product was GpppGpC. Our results indicate that the 30S ribonucleoprotein particles (informofers) as well as the proteins of the nuclear sap possess both guanylyltransferase, N7-, and 2-o-methyltransferase activities.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalMolecular Biology Reports
Issue number2
Publication statusPublished - Jun 1 1978


ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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