Methods to assess the role of poly(ADP-ribose) polymerases in regulating mitochondrial oxidation

Edit Mikó, Tünde Kovács, Tamás Fodor, Péter Bai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The impact of poly(ADP-ribose) polymerase (PARP) enzymes on cellular NAD+ has been established for almost 30 years now and its sequel, the metabolic collapse of cells upon PARP overactivation is a nearly 20-year-old observation. However, in the last decade there was an enormous blooming in the understanding of the interplay between PARPs and mitochondria. Mitochondrial activity can be assessed by a comprehensive set of methods that we aim to introduce here.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages185-200
Number of pages16
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1608
ISSN (Print)1064-3745

Keywords

  • ARTD
  • Flow cytometry
  • Mitochondria
  • Oximetry
  • PARP
  • Seahorse extracellular flux analyzer
  • Δψ

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mikó, E., Kovács, T., Fodor, T., & Bai, P. (2017). Methods to assess the role of poly(ADP-ribose) polymerases in regulating mitochondrial oxidation. In Methods in Molecular Biology (pp. 185-200). (Methods in Molecular Biology; Vol. 1608). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6993-7_13