Methods for the construction of phage-displayed libraries

Frederic A. Fellouse, G. Pál

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

In this chapter, we present different methods for making libraries of recombinant polypeptides. First, we describe how to create highly defined libraries by using synthetic DNA. Second, we present alternative approaches that can be used to introduce mutations randomly. In these cases, the position and nature of the mutations are not defined by the experimenter. Both types of methods enable the production of libraries that can be used to select polypeptides with desired functions. Finally, we describe techniques that have been developed to further increase the diversity of these libraries by either recombination or shuffling.

Original languageEnglish
Title of host publicationPhage Display in Biotechnology and Drug Discovery, Second Edition
PublisherCRC Press
Pages75-96
Number of pages22
ISBN (Electronic)9781439836507
ISBN (Print)9781439836491
DOIs
Publication statusPublished - Jan 1 2015

ASJC Scopus subject areas

  • Medicine(all)
  • Social Sciences(all)
  • Chemistry(all)

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    Fellouse, F. A., & Pál, G. (2015). Methods for the construction of phage-displayed libraries. In Phage Display in Biotechnology and Drug Discovery, Second Edition (pp. 75-96). CRC Press. https://doi.org/10.1201/b18196