Glucagon radioimmunoassay establishment with antiglucagon rabbit serum, and its difficulties are reported. Attention must be paid to the importance in the laboratory of glucagon-binding antibodies in the blood of insulin-treated diabetics. These antibodies bind the circulating endogenous glucagon, as well as 125I-glucagon. Thus their removal from the sample becomes necessary. As an example of clinical use of glucagon radioimmunoassay, the glucagon level of patients after pancreatography, that of polytraumatized and cranially injured patients is reviewed.
|Number of pages||12|
|Publication status||Published - Jan 1 1977|
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism