Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging

Gábor Orbán, Domokos Meszéna, Kinga Réka Tasnády, B. Rózsa, I. Ulbert, Gergely Márton

Research output: Contribution to journalArticle

Abstract

The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.

Original languageEnglish
Article numbere0221510
JournalPloS one
Volume14
Issue number8
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Microelectrodes
Photons
Artifacts
image analysis
Imaging techniques
Observation
Monitoring
monitoring
lasers
Microscopy
microscopy
data analysis
Microscopic examination
Lasers
methodology
Calcium
calcium
mice
photons
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging. / Orbán, Gábor; Meszéna, Domokos; Tasnády, Kinga Réka; Rózsa, B.; Ulbert, I.; Márton, Gergely.

In: PloS one, Vol. 14, No. 8, e0221510, 01.01.2019.

Research output: Contribution to journalArticle

Orbán, Gábor ; Meszéna, Domokos ; Tasnády, Kinga Réka ; Rózsa, B. ; Ulbert, I. ; Márton, Gergely. / Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging. In: PloS one. 2019 ; Vol. 14, No. 8.
@article{31caf39e679f479f888553fda573ff1d,
title = "Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging",
abstract = "The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.",
author = "G{\'a}bor Orb{\'a}n and Domokos Mesz{\'e}na and Tasn{\'a}dy, {Kinga R{\'e}ka} and B. R{\'o}zsa and I. Ulbert and Gergely M{\'a}rton",
year = "2019",
month = "1",
day = "1",
doi = "10.1371/journal.pone.0221510",
language = "English",
volume = "14",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging

AU - Orbán, Gábor

AU - Meszéna, Domokos

AU - Tasnády, Kinga Réka

AU - Rózsa, B.

AU - Ulbert, I.

AU - Márton, Gergely

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.

AB - The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy around the implanted microelectrodes. A special filtering algorithm was used for data analysis to eliminate the artefacts caused by the imaging laser. Utilization of a special filtering algorithm allowed us to detect and sort single unit activities from simultaneous two-photon imaging and electrophysiological measurement.

UR - http://www.scopus.com/inward/record.url?scp=85070946986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070946986&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0221510

DO - 10.1371/journal.pone.0221510

M3 - Article

VL - 14

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 8

M1 - e0221510

ER -