Methacholine responsiveness in mice from 2 to 8 wk of age

Elizabeth M. Bozanich, Tibor Z. Jánosi, Rachel A. Collins, Cindy Thamrin, Debra J. Turner, Z. Hantos, Peter D. Sly

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Many chronic human lung diseases have their origin in early childhood, yet most murine models used to study them utilize adult mice. An important component of the asthma phenotype is exaggerated airway responses, frequently modelled by methacholine (MCh) challenge. The present study was undertaken to characterize MCh responses in mice from 2 to 8 wk of age measuring absolute lung volume and volume-corrected respiratory mechanics as outcome variables. Female BALB/c mice aged 2, 3, 4, 6, and 8 wk were studied during cumulative intravenous MCh challenge. Following each MCh dose, absolute lung volume was measured plethysmographically at functional residual volume and during a slow inflation to 20-hPa transrespiratory pressure. Respiratory system impedance was measured continuously during the inflation maneuver and partitioned into airway and constant-phase parenchymal components by model fitting. Volume-corrected (specific) estimates of respiratory mechanics were calculated. Intravenous MCh challenge induced a predominantly airway response with no evidence of airway closure in any age group. No changes in functional residual volume were seen in mice of any age during the MCh challenge. The specific airway resistance MCh dose response curves did not show significant differences between the age groups. The results from the present study do not show systematic differences in MCh responsiveness in mice from 2 to 8 wk of age.

Original languageEnglish
Pages (from-to)542-546
Number of pages5
JournalJournal of Applied Physiology
Volume103
Issue number2
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Methacholine Chloride
Respiratory Mechanics
Residual Volume
Economic Inflation
Age Groups
Lung
Airway Resistance
Electric Impedance
Respiratory System
Lung Diseases
Asthma
Phenotype
Pressure

Keywords

  • Animal models
  • Lung volumes
  • Respiratory impedance

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Bozanich, E. M., Jánosi, T. Z., Collins, R. A., Thamrin, C., Turner, D. J., Hantos, Z., & Sly, P. D. (2007). Methacholine responsiveness in mice from 2 to 8 wk of age. Journal of Applied Physiology, 103(2), 542-546. https://doi.org/10.1152/japplphysiol.01253.2006

Methacholine responsiveness in mice from 2 to 8 wk of age. / Bozanich, Elizabeth M.; Jánosi, Tibor Z.; Collins, Rachel A.; Thamrin, Cindy; Turner, Debra J.; Hantos, Z.; Sly, Peter D.

In: Journal of Applied Physiology, Vol. 103, No. 2, 08.2007, p. 542-546.

Research output: Contribution to journalArticle

Bozanich, EM, Jánosi, TZ, Collins, RA, Thamrin, C, Turner, DJ, Hantos, Z & Sly, PD 2007, 'Methacholine responsiveness in mice from 2 to 8 wk of age', Journal of Applied Physiology, vol. 103, no. 2, pp. 542-546. https://doi.org/10.1152/japplphysiol.01253.2006
Bozanich, Elizabeth M. ; Jánosi, Tibor Z. ; Collins, Rachel A. ; Thamrin, Cindy ; Turner, Debra J. ; Hantos, Z. ; Sly, Peter D. / Methacholine responsiveness in mice from 2 to 8 wk of age. In: Journal of Applied Physiology. 2007 ; Vol. 103, No. 2. pp. 542-546.
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