Metastasis-Associated S100A4 is a specific amine donor and an activity-independent binding partner of transglutaminase-2

Béata Biri, Bence Kiss, Róbert Kiŕaly, Gitta Schlosser, Orsolya Ĺang, Ĺaszĺo Kohidai, Ĺaszĺo Fésüs, Ĺaszĺo Nyitray

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8 Citations (Scopus)


Transglutaminase-2 (TG2) is best known as a Ca2+-dependent cross-linking enzyme; however, some of its extracellular matrixrelated functions are independent of its catalytic activity and include matrix remodelling, adhesion and migration. S100A4 belongs to the Ca2+-binding EF-hand S100 protein family and acts both intra-and extra-cellularly through binding to various partners. It regulates cell migration and its overexpression is strongly associated with metastasis and poor survival in various cancers. It has recently been suggested that TG2 mediates S100A4-dependent tumour cell migration. In the present study we provide evidence that S100A4 is an interacting partner and also a specific amine donor of TG2. TG2 incorporates a glutamine donor peptide to Lys100 in the C-Terminal random coil region of S100A4. Importantly, the enzyme activity is not necessary for the interaction: S100A4 also binds to TG2 in the presence of a specific inhibitor that keeps the enzyme in an open conformation, or to an enzymatically inactive mutant. We also found that S100A4 considerably enhances TG2-mediated adhesion of A431 epithelial carcinoma cells to the extracellular matrix. This role is independent of enzyme activity and requires the open conformation of TG2.We propose that S100A4 stabilizes the open conformation of TG2, which binds to its cellsurface receptor in this state and increases cell adhesion.

Original languageEnglish
Article numberA4
Pages (from-to)31-42
Number of pages12
JournalBiochemical Journal
Issue number1
Publication statusPublished - Jan 1 2016


  • Cell Adhesion
  • Metastasis
  • Protein Cross-Linking
  • S100 Proteins
  • S100a4
  • Transglutaminase-2.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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