Polyacrylamide gel electrophoresis combined with proton induced X-ray emission spectroscopy is suitable to identify and to determine the relative amounts of protein bound metals in situ. An analysis of the hydrogenase from Thiocapsa roseopersicina has shown the feasibility of the technique and provides new insight into the relative amount as well as the intramolecular location of Fe and Ni metal atoms in this enzyme.
|Number of pages||5|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - Jul 14 1989|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology