Metal composition analysis of hydrogenase from Thiocapsa roseopersicina by proton induced X-ray emission spectroscopy

Cs Bagyinka, Z. Szokefalvi-Nagy, I. Demeter, K. L. Kovacs

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Polyacrylamide gel electrophoresis combined with proton induced X-ray emission spectroscopy is suitable to identify and to determine the relative amounts of protein bound metals in situ. An analysis of the hydrogenase from Thiocapsa roseopersicina has shown the feasibility of the technique and provides new insight into the relative amount as well as the intramolecular location of Fe and Ni metal atoms in this enzyme.

Original languageEnglish
Pages (from-to)422-426
Number of pages5
JournalBiochemical and biophysical research communications
Volume162
Issue number1
DOIs
Publication statusPublished - Jul 14 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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