Metahyphopichia laotica gen. Nov., sp. nov., a polymorphic yeast related to hyphopichia

M. Sipiczki, Walter P. Pfliegler, Silvana V B Safar, Paula B. Morais, Carlos A. Rosa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Four strains alternating between yeast and filamentous growth morphologies were isolated from flowers in two regions of Laos. In liquid environment the isolates propagated by budding and developed irregularly shaped pseudohyphae. On solid media, their yeast cells switched to hyphal growth which could return to the yeast phase by developing lateral blastoconidia. The sequences of the D1/D2 domains of the large subunit (LSU) 26S rRNA genes, the internal transcribed spacer (ITS) regions and the small subunit (SSU) 18S rRNA genes were identical in the four strains and differed from the corresponding sequences of other yeast species available in databases by at least 11 % (D1/D2), 13 % (ITS) and 7 % (SSU). In an independent project, two strains with D1/D2 and ITS sequences very similar to those of the Laotian strains were found in bark samples collected in Brazil. The six strains also differed from the closest yeast species in physiological properties, indicating that they represented a hitherto undescribed species. Phylogenetic analysis of the D1/D2 sequences, and the concatenated sequences of the SSU rRNA genes, D1/D2 domains of LSU rRNA genes as well as the protein-encoding genes ACT1 and TEF1 placed thestrains close to Hyphopichia. To reflect this position, the novel genus name Metahyphopichia gen. nov. and the novel species name Metahyphopichia laotica gen. nov., sp. nov. are proposed for them. The type strain of the type species is 11-1006T(=CBS 13022T=CCY 092-001-001T=NCAIM Y.02126T) and was isolated in Luang Prabang (Laos). MycoBank registration numbers are MB 808253 (Metahyphopichia) and MB 808254 (Metahyphopichia laotica).

Original languageEnglish
Article number001091
Pages (from-to)2550-2557
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

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yeast
rRNA Genes
Yeasts
yeasts
gene
new species
Laos
ribosomal RNA
internal transcribed spacers
Names
genes
blastospores
Growth
bark
Brazil
flower
Databases
phylogenetics
liquid
protein

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Metahyphopichia laotica gen. Nov., sp. nov., a polymorphic yeast related to hyphopichia. / Sipiczki, M.; Pfliegler, Walter P.; Safar, Silvana V B; Morais, Paula B.; Rosa, Carlos A.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 7, 001091, 01.07.2016, p. 2550-2557.

Research output: Contribution to journalArticle

Sipiczki, M. ; Pfliegler, Walter P. ; Safar, Silvana V B ; Morais, Paula B. ; Rosa, Carlos A. / Metahyphopichia laotica gen. Nov., sp. nov., a polymorphic yeast related to hyphopichia. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 7. pp. 2550-2557.
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