Metagenomika - a velünk élo′ mikroorganizmusok megismerésének új megközelítése

Translated title of the contribution: Metagenomics - A new approach to study our microbial neighbours

Krikó Eszter, Farkas Róbert, Adorján András, L. Makrai, N. Solymosi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: In our age, the information society, the computer based technologies, including the data analytical procedures are parts of each site of the life. Bioinformatics provides approaches for the knowledge gathering and update which were not imaginable two decades ago. Metagenomics is the area of bioinformatics which helps improve our knowledge about the composition of microbial species in the production or companion animals, human being or environment. Objectives: The present work describes the main steps of a metagenomics analysis. As an example, the metagenomics investigation of swine dysentery as an important animal disease is shown. Materials and methods: From three clinically diseased fattening pigs intestinal contents were sampled from three different sections of the gut (i.e. caecum, colon and rectum). All DNA was extracted from the samples, thereafter sequenced by next generation sequencing technology. The produced 1, 782, 466 reads were pooled and analysed. The reads were aligned to archea, bacteria and virus genome databases by the Kraken bioinformatics tool. The results of the alignments were visualized by the tool Krona. Results and Discussion: The presented case study helps the reader understand how metagenomics studies work and provide new professional knowledge on the microbiome of selected organs in different creatures. The proportions of the different taxa allow to have an insight to the microbial communities of the gut in the diseased animals. Beside this, a short review is presented to demonstrate the application possibilities of metagenomics in the field of animal health. Bioinformatics is an emerging area and it is very important to have knowledge -clinicians and researchers as well - about the methods, their possible results and the limitations. This work provides a simple introduction to metagenomics in the language of veterinarians.

Original languageHungarian
Pages (from-to)423-429
Number of pages7
JournalMagyar Allatorvosok Lapja
Volume140
Issue number7
Publication statusPublished - Jul 1 2018

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Metagenomics
Computational Biology
bioinformatics
Animal Diseases
Swine
digestive system
swine dysentery
Technology
Dysentery
Gastrointestinal Contents
Veterinarians
Microbiota
Pets
Archaea
animal diseases
rectum
Rectum
animal health
cecum
pets

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Metagenomika - a velünk élo′ mikroorganizmusok megismerésének új megközelítése. / Eszter, Krikó; Róbert, Farkas; András, Adorján; Makrai, L.; Solymosi, N.

In: Magyar Allatorvosok Lapja, Vol. 140, No. 7, 01.07.2018, p. 423-429.

Research output: Contribution to journalArticle

Eszter, Krikó ; Róbert, Farkas ; András, Adorján ; Makrai, L. ; Solymosi, N. / Metagenomika - a velünk élo′ mikroorganizmusok megismerésének új megközelítése. In: Magyar Allatorvosok Lapja. 2018 ; Vol. 140, No. 7. pp. 423-429.
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