Metabolism of inositol-1,3,4,6-tetrakisphosphate to inositol pentakisphosphate in adrenal glomerulosa cells

L. Hunyady, Albert J. Baukal, Gaetan Guillemette, Tamás Balla, Kevin J. Catt

Research output: Contribution to journalArticle

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Abstract

Angiotensin II stimulates rapid formation of inositol-1,4,5-triphosphate (Ins-1,4,5-P3) in bovine adrenal glomerulosa cells. In addition to being rapidly metabolized to lower inositol phosphates, Ins-1,4,5-P3 is converted to Ins-1,3,4,5-tetrakisphosphate (Ins-1,3,4,5-P4) and Ins-1,3,4-P3 which is in turn phosphorylated to a further Ins-P4 isomer, namely Ins-1,3,4,6-P4. In bovine adrenocortical cytosol [3H]Ins-1,3,4,5-P4 and [3H]Ins-1,3,4-P3 were converted to Ins-1,3,4,6-P4 and inositol pentakisphosphate (Ins-P5) in a metabolic sequence suggesting that unlike Ins-1,3,4,5-P4, Ins-1,3,4,6-P4 is a direct precursor of Ins-P5. Consistent with this assumption, [3H]Ins-1,3,4,6-P4 was converted to Ins-P5 in electropermeabilized adrenal glomerulosa cells. These findings demonstrate that Ins-1,3,4,6-P4 is an intermediate link between InsP3 metabolism and the higher inositol phosphates detected in several tissues.

Original languageEnglish
Pages (from-to)1247-1252
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume157
Issue number3
DOIs
Publication statusPublished - Dec 30 1988

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Zona Glomerulosa
Metabolism
Inositol Phosphates
Inositol 1,4,5-Trisphosphate
Angiotensin II
Isomers
Cytosol
inositol pentaphosphate
inositol-1,3,4,6-tetrakisphosphate
Tissue
inositol-1,3,4,5-tetrakisphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Metabolism of inositol-1,3,4,6-tetrakisphosphate to inositol pentakisphosphate in adrenal glomerulosa cells. / Hunyady, L.; Baukal, Albert J.; Guillemette, Gaetan; Balla, Tamás; Catt, Kevin J.

In: Biochemical and Biophysical Research Communications, Vol. 157, No. 3, 30.12.1988, p. 1247-1252.

Research output: Contribution to journalArticle

Hunyady, L. ; Baukal, Albert J. ; Guillemette, Gaetan ; Balla, Tamás ; Catt, Kevin J. / Metabolism of inositol-1,3,4,6-tetrakisphosphate to inositol pentakisphosphate in adrenal glomerulosa cells. In: Biochemical and Biophysical Research Communications. 1988 ; Vol. 157, No. 3. pp. 1247-1252.
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N2 - Angiotensin II stimulates rapid formation of inositol-1,4,5-triphosphate (Ins-1,4,5-P3) in bovine adrenal glomerulosa cells. In addition to being rapidly metabolized to lower inositol phosphates, Ins-1,4,5-P3 is converted to Ins-1,3,4,5-tetrakisphosphate (Ins-1,3,4,5-P4) and Ins-1,3,4-P3 which is in turn phosphorylated to a further Ins-P4 isomer, namely Ins-1,3,4,6-P4. In bovine adrenocortical cytosol [3H]Ins-1,3,4,5-P4 and [3H]Ins-1,3,4-P3 were converted to Ins-1,3,4,6-P4 and inositol pentakisphosphate (Ins-P5) in a metabolic sequence suggesting that unlike Ins-1,3,4,5-P4, Ins-1,3,4,6-P4 is a direct precursor of Ins-P5. Consistent with this assumption, [3H]Ins-1,3,4,6-P4 was converted to Ins-P5 in electropermeabilized adrenal glomerulosa cells. These findings demonstrate that Ins-1,3,4,6-P4 is an intermediate link between InsP3 metabolism and the higher inositol phosphates detected in several tissues.

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