Metabolic activity and phylogenetic diversity of reed (Phragmites australis) periphyton bacterial communities in a Hungarian shallow soda lake

A. Borsodi, Anna Rusznyák, Piroska Molnár, Péter Vladár, Mária N. Reskóné, E. Tóth, Rita Sipos, Gábor Gedeon, K. Márialigeti

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In the present study, the species composition and potential metabolic activities of bacterial communities of reed Phragmites australis (Cav.) (Trin. ex Steudel) periphyton from Lake Velencei were studied by cultivation-based and metabolic fingerprinting methods. Serially diluted spring biofilm samples were used to test the community-level physiological profiling (CLPP) using BIOLOG microplates, and for plating onto different media. On the basis of their morphological, biochemical, and physiological test results, 173 strains were clustered by numerical analysis. Representatives of amplified ribosomal DNA restriction analysis (ARDRA) groups were identified by their 16S rDNA sequence comparison. Based on the results of the CLPP investigations, regional differences were detected among the utilized substrate numbers and types, parallel with the increase in incubation time. The phenotypic test results of the strains showed considerable variability with respect to the sampling sites and the media used for cultivation. The most frequently isolated strains were identified as members of genera Agrobacterium, Pseudomonas (P. anguilliseptica, P. marginalis, P. alcaligenes, P. fragi) with aerobic or facultative anaerobic respiratory metabolism, and the species Aeromonas sobria and A. veronii with strong facultative fermentative metabolism. Other strains were identified as Gram-positive Arthrobacter, Bacillus, and Kocuria species. The rarely isolated strains were members of β-Proteobacteria (Acidovorax, Delftia, Hydrogenophaga, and Rhodoferax), γ-Proteobacteria (Psychrobacter and Shewanella), low G + C Gram-positives (Brevibacillus, Paenibacillus, and Exiguobacterium) and high G + C Gram-positives (Aureobacterium and Microbacterium).

Original languageEnglish
Pages (from-to)612-620
Number of pages9
JournalMicrobial Ecology
Volume53
Issue number4
DOIs
Publication statusPublished - May 2007

Fingerprint

periphyton
Phragmites australis
Comamonadaceae
Lakes
bacterial communities
Proteobacteria
Ribosomal DNA
phylogenetics
lakes
Delftia
Psychrobacter
phylogeny
Brevibacillus
lake
metabolism
Paenibacillus
Shewanella
Anaerobiosis
Alcaligenes
Arthrobacter

ASJC Scopus subject areas

  • Aquatic Science
  • Applied Microbiology and Biotechnology
  • Ecology
  • Microbiology

Cite this

Metabolic activity and phylogenetic diversity of reed (Phragmites australis) periphyton bacterial communities in a Hungarian shallow soda lake. / Borsodi, A.; Rusznyák, Anna; Molnár, Piroska; Vladár, Péter; Reskóné, Mária N.; Tóth, E.; Sipos, Rita; Gedeon, Gábor; Márialigeti, K.

In: Microbial Ecology, Vol. 53, No. 4, 05.2007, p. 612-620.

Research output: Contribution to journalArticle

Borsodi, A. ; Rusznyák, Anna ; Molnár, Piroska ; Vladár, Péter ; Reskóné, Mária N. ; Tóth, E. ; Sipos, Rita ; Gedeon, Gábor ; Márialigeti, K. / Metabolic activity and phylogenetic diversity of reed (Phragmites australis) periphyton bacterial communities in a Hungarian shallow soda lake. In: Microbial Ecology. 2007 ; Vol. 53, No. 4. pp. 612-620.
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AU - Reskóné, Mária N.

AU - Tóth, E.

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