Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells

K. Rácz, I. Varga, E. Gláz, R. Kiss, S. Vida, G. Lada, K. di Gleria, K. Medzihradszky, K. Lichtwald, P. Vecsei

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In vitro application of the morphinomimetic metenkephalin resulted in inhibition of mineralocorticoid production by aldosterone-producing adenomas. Aldosterone, deoxycorticosterone, and corticosterone production by adrenocortical cells isolated from aldosteronomas has been studied under basal conditions and after stimulation with ACTH-(1-24). The blocking effect of met-enkephalin on the rate of aldosterone, deoxycorticosterone, and corticosterone release was significant at a concentration as low as 10-11 M (P <0.001, P <0.01, and P <0.001, respectively). Dose-dependent inhibition of steroid biosynthesis became more apparent with increasing amounts of met-enkephalin in the incubation medium (10-11-10-5 M); at a concentration of 10-5 M, met-enkephalin decreased the production of aldosterone by 45%, that of deoxycorticosterone by 51%, and that of corticosterone by 44%. Increased steroid biosynthesis stimulated by ACTH-(1-24) was also significantly blocked by met-enkephalin. In a concentration of 10-5 M, met-enkephalin produced significant decreases in aldosterone (P <0.001), deoxycorticosterone (P <0.05), and corticosterone (P <0.001) production compared to the peak values obtained after stimulation with 0.85 x 10-10 M ACTH-(1-24). These data allow us to conclude that the inhibitory effect of met-enkephalin on mineralocorticoid production exerted at the level of the adrenals might be complementary to the factor(s) thought to be involved in the regulation of adrenal steroid production, playing a role similar to that of the biogenic amines originating in the adrenal medulla and regulating the adrenal cortex by a peripheral neurohumoral paracrine mechanism.

Original languageEnglish
Pages (from-to)656-660
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume54
Issue number3
Publication statusPublished - 1982

Fingerprint

Methionine Enkephalin
Mineralocorticoids
Aldosterone
Desoxycorticosterone
Corticosterone
Cosyntropin
Biosynthesis
Steroids
Adrenal Medulla
Biogenic Amines
Adrenal Cortex
Adenoma

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells. / Rácz, K.; Varga, I.; Gláz, E.; Kiss, R.; Vida, S.; Lada, G.; di Gleria, K.; Medzihradszky, K.; Lichtwald, K.; Vecsei, P.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 54, No. 3, 1982, p. 656-660.

Research output: Contribution to journalArticle

Rácz, K, Varga, I, Gláz, E, Kiss, R, Vida, S, Lada, G, di Gleria, K, Medzihradszky, K, Lichtwald, K & Vecsei, P 1982, 'Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells', Journal of Clinical Endocrinology and Metabolism, vol. 54, no. 3, pp. 656-660.
Rácz, K. ; Varga, I. ; Gláz, E. ; Kiss, R. ; Vida, S. ; Lada, G. ; di Gleria, K. ; Medzihradszky, K. ; Lichtwald, K. ; Vecsei, P. / Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells. In: Journal of Clinical Endocrinology and Metabolism. 1982 ; Vol. 54, No. 3. pp. 656-660.
@article{070dfdbe0e9f4d6bb11e877a8607e01d,
title = "Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells",
abstract = "In vitro application of the morphinomimetic metenkephalin resulted in inhibition of mineralocorticoid production by aldosterone-producing adenomas. Aldosterone, deoxycorticosterone, and corticosterone production by adrenocortical cells isolated from aldosteronomas has been studied under basal conditions and after stimulation with ACTH-(1-24). The blocking effect of met-enkephalin on the rate of aldosterone, deoxycorticosterone, and corticosterone release was significant at a concentration as low as 10-11 M (P <0.001, P <0.01, and P <0.001, respectively). Dose-dependent inhibition of steroid biosynthesis became more apparent with increasing amounts of met-enkephalin in the incubation medium (10-11-10-5 M); at a concentration of 10-5 M, met-enkephalin decreased the production of aldosterone by 45{\%}, that of deoxycorticosterone by 51{\%}, and that of corticosterone by 44{\%}. Increased steroid biosynthesis stimulated by ACTH-(1-24) was also significantly blocked by met-enkephalin. In a concentration of 10-5 M, met-enkephalin produced significant decreases in aldosterone (P <0.001), deoxycorticosterone (P <0.05), and corticosterone (P <0.001) production compared to the peak values obtained after stimulation with 0.85 x 10-10 M ACTH-(1-24). These data allow us to conclude that the inhibitory effect of met-enkephalin on mineralocorticoid production exerted at the level of the adrenals might be complementary to the factor(s) thought to be involved in the regulation of adrenal steroid production, playing a role similar to that of the biogenic amines originating in the adrenal medulla and regulating the adrenal cortex by a peripheral neurohumoral paracrine mechanism.",
author = "K. R{\'a}cz and I. Varga and E. Gl{\'a}z and R. Kiss and S. Vida and G. Lada and {di Gleria}, K. and K. Medzihradszky and K. Lichtwald and P. Vecsei",
year = "1982",
language = "English",
volume = "54",
pages = "656--660",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Met-enkephalin inhibits mineralocorticoid production in isolated human aldosteronoma cells

AU - Rácz, K.

AU - Varga, I.

AU - Gláz, E.

AU - Kiss, R.

AU - Vida, S.

AU - Lada, G.

AU - di Gleria, K.

AU - Medzihradszky, K.

AU - Lichtwald, K.

AU - Vecsei, P.

PY - 1982

Y1 - 1982

N2 - In vitro application of the morphinomimetic metenkephalin resulted in inhibition of mineralocorticoid production by aldosterone-producing adenomas. Aldosterone, deoxycorticosterone, and corticosterone production by adrenocortical cells isolated from aldosteronomas has been studied under basal conditions and after stimulation with ACTH-(1-24). The blocking effect of met-enkephalin on the rate of aldosterone, deoxycorticosterone, and corticosterone release was significant at a concentration as low as 10-11 M (P <0.001, P <0.01, and P <0.001, respectively). Dose-dependent inhibition of steroid biosynthesis became more apparent with increasing amounts of met-enkephalin in the incubation medium (10-11-10-5 M); at a concentration of 10-5 M, met-enkephalin decreased the production of aldosterone by 45%, that of deoxycorticosterone by 51%, and that of corticosterone by 44%. Increased steroid biosynthesis stimulated by ACTH-(1-24) was also significantly blocked by met-enkephalin. In a concentration of 10-5 M, met-enkephalin produced significant decreases in aldosterone (P <0.001), deoxycorticosterone (P <0.05), and corticosterone (P <0.001) production compared to the peak values obtained after stimulation with 0.85 x 10-10 M ACTH-(1-24). These data allow us to conclude that the inhibitory effect of met-enkephalin on mineralocorticoid production exerted at the level of the adrenals might be complementary to the factor(s) thought to be involved in the regulation of adrenal steroid production, playing a role similar to that of the biogenic amines originating in the adrenal medulla and regulating the adrenal cortex by a peripheral neurohumoral paracrine mechanism.

AB - In vitro application of the morphinomimetic metenkephalin resulted in inhibition of mineralocorticoid production by aldosterone-producing adenomas. Aldosterone, deoxycorticosterone, and corticosterone production by adrenocortical cells isolated from aldosteronomas has been studied under basal conditions and after stimulation with ACTH-(1-24). The blocking effect of met-enkephalin on the rate of aldosterone, deoxycorticosterone, and corticosterone release was significant at a concentration as low as 10-11 M (P <0.001, P <0.01, and P <0.001, respectively). Dose-dependent inhibition of steroid biosynthesis became more apparent with increasing amounts of met-enkephalin in the incubation medium (10-11-10-5 M); at a concentration of 10-5 M, met-enkephalin decreased the production of aldosterone by 45%, that of deoxycorticosterone by 51%, and that of corticosterone by 44%. Increased steroid biosynthesis stimulated by ACTH-(1-24) was also significantly blocked by met-enkephalin. In a concentration of 10-5 M, met-enkephalin produced significant decreases in aldosterone (P <0.001), deoxycorticosterone (P <0.05), and corticosterone (P <0.001) production compared to the peak values obtained after stimulation with 0.85 x 10-10 M ACTH-(1-24). These data allow us to conclude that the inhibitory effect of met-enkephalin on mineralocorticoid production exerted at the level of the adrenals might be complementary to the factor(s) thought to be involved in the regulation of adrenal steroid production, playing a role similar to that of the biogenic amines originating in the adrenal medulla and regulating the adrenal cortex by a peripheral neurohumoral paracrine mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0020382386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020382386&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 656

EP - 660

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 3

ER -