Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

Sándor Katz, Petra Balogh, A. Kiss

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Peritoneal cell suspension is composed of heterogeneous cell population. Macrophages are the most numerous cells among them. They can originate from different sources and can be resident, exudate and elicited. When we used Freund's adjuvant to elicit peritoneal macrophages, cells having large amount of caveolae on their plasma membrane appeared in the peritoneal wash. The number of these caveolae-rich cells increased by the time of the Freund's adjuvant treatment. Although their morphology was different form from the common macrophages, they were labelled with pan-macrophage antibodies. As the origin of these cells is unknown in this work, we tried to find out where they can originate from. Our interest turned towards the mesothelial cells. We found that the adjuvant treatment resulted in significant morphological changes in these cells and stimulate them to leave the surface of the mesentery. By the time of the adjuvant treatment, the macrophage markers expression increased in the mesothelial cells and more cells were found to detach from the mesentery. These results strongly suggest that under special stimuli mesothelial cells can leave the mesentery and differentiate into phagocytotic (macrophage-like) cells. These data raises the idea that mesothelial cells might not entirely differentiated and represent a multipotential cell lineage. To study whether this is the case we used anti-nestin antibody, which is a specific marker for multifunctional, multi-lineage progenitor cells. Mesothelial cells showed strong labelling with this antibody indicating that these cells really represent a 'young', not entirely differentiated cell population.

Original languageEnglish
Pages (from-to)782-793
Number of pages12
JournalAPMIS
Volume119
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Mesentery
Macrophages
Caveolae
Freund's Adjuvant
Nestin
Antibodies
Peritoneal Macrophages
Cell Lineage
Exudates and Transudates

Keywords

  • Caveolae
  • Macrophages
  • Mesothelial cells
  • Multi-lineage progenitor cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)
  • Immunology and Allergy

Cite this

Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells. / Katz, Sándor; Balogh, Petra; Kiss, A.

In: APMIS, Vol. 119, No. 11, 11.2011, p. 782-793.

Research output: Contribution to journalArticle

Katz, Sándor ; Balogh, Petra ; Kiss, A. / Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells. In: APMIS. 2011 ; Vol. 119, No. 11. pp. 782-793.
@article{c18dec4057074d4fa1c550a932c6a073,
title = "Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells",
abstract = "Peritoneal cell suspension is composed of heterogeneous cell population. Macrophages are the most numerous cells among them. They can originate from different sources and can be resident, exudate and elicited. When we used Freund's adjuvant to elicit peritoneal macrophages, cells having large amount of caveolae on their plasma membrane appeared in the peritoneal wash. The number of these caveolae-rich cells increased by the time of the Freund's adjuvant treatment. Although their morphology was different form from the common macrophages, they were labelled with pan-macrophage antibodies. As the origin of these cells is unknown in this work, we tried to find out where they can originate from. Our interest turned towards the mesothelial cells. We found that the adjuvant treatment resulted in significant morphological changes in these cells and stimulate them to leave the surface of the mesentery. By the time of the adjuvant treatment, the macrophage markers expression increased in the mesothelial cells and more cells were found to detach from the mesentery. These results strongly suggest that under special stimuli mesothelial cells can leave the mesentery and differentiate into phagocytotic (macrophage-like) cells. These data raises the idea that mesothelial cells might not entirely differentiated and represent a multipotential cell lineage. To study whether this is the case we used anti-nestin antibody, which is a specific marker for multifunctional, multi-lineage progenitor cells. Mesothelial cells showed strong labelling with this antibody indicating that these cells really represent a 'young', not entirely differentiated cell population.",
keywords = "Caveolae, Macrophages, Mesothelial cells, Multi-lineage progenitor cells",
author = "S{\'a}ndor Katz and Petra Balogh and A. Kiss",
year = "2011",
month = "11",
doi = "10.1111/j.1600-0463.2011.02803.x",
language = "English",
volume = "119",
pages = "782--793",
journal = "Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology",
issn = "0365-5555",
publisher = "Wiley-Blackwell",
number = "11",

}

TY - JOUR

T1 - Mesothelial cells can detach from the mesentery and differentiate into macrophage-like cells

AU - Katz, Sándor

AU - Balogh, Petra

AU - Kiss, A.

PY - 2011/11

Y1 - 2011/11

N2 - Peritoneal cell suspension is composed of heterogeneous cell population. Macrophages are the most numerous cells among them. They can originate from different sources and can be resident, exudate and elicited. When we used Freund's adjuvant to elicit peritoneal macrophages, cells having large amount of caveolae on their plasma membrane appeared in the peritoneal wash. The number of these caveolae-rich cells increased by the time of the Freund's adjuvant treatment. Although their morphology was different form from the common macrophages, they were labelled with pan-macrophage antibodies. As the origin of these cells is unknown in this work, we tried to find out where they can originate from. Our interest turned towards the mesothelial cells. We found that the adjuvant treatment resulted in significant morphological changes in these cells and stimulate them to leave the surface of the mesentery. By the time of the adjuvant treatment, the macrophage markers expression increased in the mesothelial cells and more cells were found to detach from the mesentery. These results strongly suggest that under special stimuli mesothelial cells can leave the mesentery and differentiate into phagocytotic (macrophage-like) cells. These data raises the idea that mesothelial cells might not entirely differentiated and represent a multipotential cell lineage. To study whether this is the case we used anti-nestin antibody, which is a specific marker for multifunctional, multi-lineage progenitor cells. Mesothelial cells showed strong labelling with this antibody indicating that these cells really represent a 'young', not entirely differentiated cell population.

AB - Peritoneal cell suspension is composed of heterogeneous cell population. Macrophages are the most numerous cells among them. They can originate from different sources and can be resident, exudate and elicited. When we used Freund's adjuvant to elicit peritoneal macrophages, cells having large amount of caveolae on their plasma membrane appeared in the peritoneal wash. The number of these caveolae-rich cells increased by the time of the Freund's adjuvant treatment. Although their morphology was different form from the common macrophages, they were labelled with pan-macrophage antibodies. As the origin of these cells is unknown in this work, we tried to find out where they can originate from. Our interest turned towards the mesothelial cells. We found that the adjuvant treatment resulted in significant morphological changes in these cells and stimulate them to leave the surface of the mesentery. By the time of the adjuvant treatment, the macrophage markers expression increased in the mesothelial cells and more cells were found to detach from the mesentery. These results strongly suggest that under special stimuli mesothelial cells can leave the mesentery and differentiate into phagocytotic (macrophage-like) cells. These data raises the idea that mesothelial cells might not entirely differentiated and represent a multipotential cell lineage. To study whether this is the case we used anti-nestin antibody, which is a specific marker for multifunctional, multi-lineage progenitor cells. Mesothelial cells showed strong labelling with this antibody indicating that these cells really represent a 'young', not entirely differentiated cell population.

KW - Caveolae

KW - Macrophages

KW - Mesothelial cells

KW - Multi-lineage progenitor cells

UR - http://www.scopus.com/inward/record.url?scp=80054122726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054122726&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0463.2011.02803.x

DO - 10.1111/j.1600-0463.2011.02803.x

M3 - Article

C2 - 21995632

AN - SCOPUS:80054122726

VL - 119

SP - 782

EP - 793

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section B Microbiology

SN - 0365-5555

IS - 11

ER -