Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth

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18 Citations (Scopus)

Abstract

Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.

Original languageEnglish
Pages (from-to)474-480
Number of pages7
JournalBiochemical and biophysical research communications
Volume414
Issue number3
DOIs
Publication statusPublished - Oct 28 2011

Keywords

  • Feeder cell
  • Human embryonic stem cells
  • Immunosuppressive feature
  • Mesenchymal stem cell like (MSCl) cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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