Mercury inhibits the activity of the NADPH

protochlorophyllide oxidoreductase (POR)

K. Lenti, F. Fodor, B. Böddi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of Hg++ was studied on the arrangement and photoactivity of NADPH:protochlorophyllide oxidoreductase (POR) in homogenates of dark-grown wheat (Triticum aestivum L.) leaves. 77 K fluorescence emission spectra of the homogenates were recorded before and after the irradiation of the homogenates and the spectra were deconvoluted into Gaussian components. The mercury treatment caused a precipitation of the membrane particles, which was followed by a remarkable decrease of the fluorescence yield. 10-3 M Hg++ decreased the ratio of the 655 nm-emitting protochlorophyllide (Pchlide) form to the 633 nm-emitting form. 10-2 M Hg++ shifted the short wavelength band to 629-630 nm and a 655 nm form was observed which was inactive on irradiation. This inhibition may be caused by serious alteration of the enzyme structure resulting in the trans-localisation of NADPH within the active site of POR.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalPhotosynthetica
Volume40
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

protochlorophyllide reductase
Protochlorophyllide
protochlorophyllides
oxidoreductases
Mercury
NADP (coenzyme)
mercury
Triticum
Fluorescence
irradiation
NADP
fluorescence
Catalytic Domain
Oxidoreductases
active sites
Membranes
wavelengths
Enzymes
Triticum aestivum
wheat

Keywords

  • Chlorophyll formation
  • Fluorescence emission spectra
  • Heavy metal
  • Hg
  • Protochlorophyllide
  • Triticum
  • Wheat

ASJC Scopus subject areas

  • Plant Science

Cite this

Mercury inhibits the activity of the NADPH : protochlorophyllide oxidoreductase (POR). / Lenti, K.; Fodor, F.; Böddi, B.

In: Photosynthetica, Vol. 40, No. 1, 2002, p. 145-151.

Research output: Contribution to journalArticle

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